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Related Concept Videos

Complement System01:27

Complement System

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The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
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Complementation Tests00:49

Complementation Tests

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A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
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Updated: Nov 25, 2025

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research
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Quantitative multiplex profiling of the complement system to diagnose complement-mediated diseases.

Esther Willems1,2,3, Laura Lorés-Motta4, Andrea Zanichelli5

  • 1Laboratory of Medical Immunology Department of Laboratory Medicine Radboud Institute for Molecular Life Sciences Radboud University Medical Center Nijmegen The Netherlands.

Clinical & Translational Immunology
|December 15, 2020
PubMed
Summary
This summary is machine-generated.

A novel complementomics approach accurately identifies complement deficiencies by profiling circulating complement proteins. This method reveals complex pathway interactions, improving diagnosis and care for complement-mediated diseases.

Keywords:
complement deficienciescomplement systemcomplementomicscomplement‐mediated diseasesmultiplex targeted mass spectrometrypathway analysis

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Area of Science:

  • Immunology
  • Proteomics
  • Clinical Diagnostics

Background:

  • Complement deficiencies present diagnostic challenges due to varied symptoms and complex testing.
  • A comprehensive understanding of complement protein interactions is crucial for diagnosing and managing related diseases.

Purpose of the Study:

  • To apply a novel 'complementomics' approach for improved diagnosis of complement deficiencies.
  • To investigate the impact of complement deficiencies on circulating complement protein levels.
  • To explore the clinical utility of mass spectrometry-based complement profiling.

Main Methods:

  • Quantitative multiplex mass spectrometry was used to analyze 44 peptides, profiling 34 complement proteins.
  • The study included 40 healthy controls and 83 individuals with diagnosed or potential complement protein deficiencies.
  • The assay was applied to individuals with deficiencies in 14 different complement proteins.

Main Results:

  • Complementomics confirmed protein absence in deficient patients and identified additional deficiencies.
  • Partial depletion of upstream and downstream complement proteins was observed, particularly in patients with complement inhibitor deficiencies.
  • The approach demonstrated added value in diagnosing three patients with previously ill-defined complement deficiencies.

Conclusions:

  • Profiling circulating complement proteins offers a comprehensive readout for various complement deficiencies.
  • The complementomics approach provides insights into the functional interplay of complement proteins.
  • This enhances understanding of disease phenotypes and improves patient care for complement-mediated diseases.