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Updated: Nov 25, 2025

High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
RNA-Seq transcriptome data of human cells infected with influenza A/Puerto Rico/8/1934 (H1N1) virus
Evgenii Zhuravlev1, Mariia Sergeeva1,2, Sergey Malanin3
1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Abstract:
Human influenza remains a serious public health problem. This data article reports the transcriptome analysis data of human cell lines infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. Mock-infected cells were included as controls. Human embryonic fibroblasts (MRC-5) and immortalized cell lines (A549, HEK293FT, WI-38 VA-13) were selected for RNA sequencing using Illumina NextSeq500 platform. Raw data were applied to the bioinformatic pipeline, which includes quality control with FastQC and MultiQC, adapter and quality trimming with Cutadapt, filtering to the genome of influenza A with STAR, transcript quantification with Salmon tool (GRCh38_RefSeq_Transcripts). Differential expressed genes were identified using R package DESeq2 with FDR-adjusted p-value < 0.001 and absolute value of log2(FC) > 1. Lists of differentially expressed genes is provided. The raw and processed RNA-seq data presented in this article were deposited to the European Nucleotide Archive via the ArrayExpress partner repository with the dataset accession number E-MTAB-9511 .
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