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Manufacture and Drug Delivery Applications of Silk Nanoparticles
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Silk Nanoparticle Manufacture in Semi-Batch Format.

Saphia A L Matthew1, John D Totten1,2, Suttinee Phuagkhaopong1

  • 1Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, U.K.

ACS Biomaterials Science & Engineering
|December 15, 2020
PubMed
Summary
This summary is machine-generated.

A new semi-batch method for fabricating silk nanoparticles using drop-by-drop nanoprecipitation improves quality and efficiency. This accessible platform enhances control over nanoparticle production for drug delivery applications.

Keywords:
biopolymerdesolvationnanoparticlenanoprecipitationsilk fibroin

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Chemical Engineering

Background:

  • Silk nanoparticles are promising for biomedical uses, particularly drug delivery.
  • Bottom-up fabrication methods like nanoprecipitation offer superior nanoparticle quality compared to top-down approaches.
  • Controlling critical quality attributes in nanoparticle manufacture is essential for consistent performance.

Purpose of the Study:

  • To develop a simple, scalable, semi-batch method for silk nanoparticle fabrication using drop-by-drop nanoprecipitation.
  • To investigate the impact of key process parameters on nanoparticle characteristics.
  • To establish a reproducible and efficient platform for silk nanoparticle production.

Main Methods:

  • A semi-batch, drop-by-drop nanoprecipitation technique was employed at the lab scale.
  • Stirring rate and dropping height were systematically varied to assess their effects.
  • Nanoparticle size, yield, morphology, and stability were analyzed.
  • Standing time in mother liquor was evaluated for its impact on nanoparticle growth.

Main Results:

  • The stirring rate significantly influenced nanoparticle size and yield (optimal at 200 rpm).
  • Dropping height directly affected nanoparticle yield.
  • Nanoparticles exhibited consistent sizes (104–134 nm), spherical morphology, and high β-sheet content.
  • Manufactured silk nanoparticles remained stable in aqueous media for over one month at 4 °C.

Conclusions:

  • The developed semi-batch drop-by-drop method offers improved mixing efficiency and reduced variation in silk nanoparticle production.
  • This accessible, higher-throughput platform facilitates standardization of critical parameters for silk nanoparticle drug delivery systems.
  • The method provides a reliable approach for producing high-quality, stable silk nanoparticles.