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Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

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Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
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Induced Pluripotent Stem Cells01:13

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
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iPS Cell Differentiation01:22

iPS Cell Differentiation

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The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
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Related Experiment Video

Updated: Nov 25, 2025

Generation and Characterization of Human Induced Pluripotent Stem Cell-derived Astrocytes Lacking Fragile X Messenger Ribonucleoprotein
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Generation and Characterization of Human Induced Pluripotent Stem Cell-derived Astrocytes Lacking Fragile X Messenger Ribonucleoprotein

Published on: June 6, 2025

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Human Astrocytes Model Derived from Induced Pluripotent Stem Cells.

Nicolas Leventoux1, Satoru Morimoto1, Kent Imaizumi1

  • 1Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.

Cells
|December 16, 2020
PubMed
Summary

Researchers developed a new method to generate astrocyte cells from induced pluripotent stem cells (iPSCs). These iPSC-derived astrocytes (iPasts) are functional and can be used for disease modeling in neurodegenerative conditions.

Keywords:
astrocytescell culturedisease modelingiPSCneurodegenerative diseases

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Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons
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Area of Science:

  • Stem cell biology
  • Neuroscience
  • Cellular and molecular medicine

Background:

  • Induced pluripotent stem cell (iPSC)-based disease modeling is crucial for understanding neurodegenerative diseases.
  • Current iPSC models primarily focus on neurons due to underdeveloped protocols for generating astrocytes.
  • Astrocytes play a significant role in neurodegeneration, highlighting the need for iPSC-derived astrocyte models.

Purpose of the Study:

  • To establish an efficient protocol for generating functional astrocytes from iPSCs (iPasts).
  • To characterize iPasts for their potential in disease modeling.
  • To investigate the utility of iPasts in studying astrocyte contributions to neurodegenerative diseases.

Main Methods:

  • Development of a novel protocol for iPSC differentiation into astrocytes.
  • Comprehensive characterization of iPasts using RNA and protein expression analysis.
  • Functional assays including calcium dynamics, glutamate uptake, and co-culture with neurons.

Main Results:

  • Successfully generated iPSC-derived astrocytes (iPasts) with an efficient protocol.
  • iPasts demonstrated comparable calcium dynamics and glutamate uptake to primary human astrocytes.
  • Co-culture of iPasts with neurons promoted neuronal synaptic maturation.

Conclusions:

  • The established protocol provides a reliable method for generating functional iPSC-derived astrocytes.
  • iPasts are suitable for in vitro modeling of astrocyte-related disease phenotypes.
  • This advancement facilitates further research into the role of astrocytes in neurodegenerative diseases.