Evidence of Hepatitis E Virus in Goat and Sheep Milk

Hepatitis E virus (HEV) is the etiological agent behind hepatitis E infection. Domestic pigs and wild boars are the main animal reservoirs of HEV. Very few papers describe HEV infection in goats and sheep. As the data pertaining to the presence of HEV virus in the milk of small ruminants in Europe are lacking, the aim of this paper was to examine a representative number of milk samples from these animals. The detection of HEV genome (HEV RNA) was performed using reverse transcriptase real-time polymerase chain reaction (RT-qPCR). HEV RNA was found in 2.8% of the examined samples. Positivity ranged from 10¹ to 10³ genome equivalents/mL (GE/mL) with a median of 9.99 × 10² GE/mL. On the basis of these results, the milk of small ruminants could represent a source of HEV infection to consumers.