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Expression of Circulating MicroRNAs Linked to Bone Metabolism in Chronic Kidney Disease-Mineral and Bone Disorder

Maria P Yavropoulou1,2, Vasilios Vaios3, Polyzois Makras2

  • 1Endocrinology Unit, The First Department of Propaedeutic and Internal Medicine, Medical School, National and Kapodistrian University of Athens, GR 11527 Athens, Greece.

Biomedicines
|December 16, 2020
PubMed

Insights

MicroRNAs (miRNAs) are linked to bone loss in chronic kidney disease-mineral and bone disorder (CKD-MBD). This study found significantly lower levels of specific miRNAs in CKD-MBD patients, suggesting their role in the condition.

Area of Science:

  • Nephrology
  • Endocrinology
  • Molecular Biology

Background:

  • Chronic kidney disease-mineral and bone disorder (CKD-MBD) involves complex pathophysiology.
  • Emerging research highlights a connection between microRNAs (miRNAs) and bone metabolism.
  • Understanding miRNA expression in CKD-MBD is crucial for elucidating bone loss mechanisms.

Purpose of the Study:

  • To investigate the expression profiles of specific miRNAs in patients with CKD-MBD.
  • To explore the relationship between miRNA expression, bone mineral density (BMD), and trabecular bone score (TBS).
  • To assess the diagnostic potential of miRNAs in identifying CKD-MBD-related osteoporosis.

Main Methods:

  • A case-control study involving 30 CKD-MBD patients and 30 healthy controls.
  • Bone mineral density (BMD) and trabecular bone score (TBS) assessed via dual X-ray absorptiometry.
  • Quantitative analysis of selected miRNAs (hsa-miRNA-21-5p, hsa-miRNA-23a-3p, hsa-miRNA-124-3p, etc.) using RT-qPCR.

Main Results:

  • CKD-MBD patients exhibited significantly lower BMD compared to controls.
  • Reduced expression of miRNA-21-5p (15-fold), miRNA-124-3p (6-fold), and miRNA-23a-3p (4-fold) was observed in CKD-MBD patients.
  • MiRNA-23a-3p inversely correlated with TBS, and miRNA-124-3p showed high sensitivity and specificity for detecting osteoporosis in CKD patients.

Conclusions:

  • Serum miRNA expression, particularly involving osteoblast- and osteoclast-related miRNAs, is significantly altered in CKD-MBD.
  • Specific miRNAs (miRNA-21-5p, miRNA-124-3p, miRNA-23a-3p) are potential biomarkers for bone loss in CKD-MBD.
  • MiRNA-124-3p may serve as a valuable diagnostic marker for identifying osteoporosis in CKD patients.
Keywords:
CKD-MBDcirculating microRNAsend stage renal diseaseosteoporosistrabecular bone score

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