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Quantitative Analysis01:12

Quantitative Analysis

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Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
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Species Quantification in Complex Herbal Formulas-Vector Control Quantitative Analysis as a New Method.

Bo Zhao1,2, Chao Xiong2,3, Jingjian Li1

  • 1College of Pharmacy, Guilin Medical University, Guilin, China.

Frontiers in Pharmacology
|December 16, 2020
PubMed
Summary
This summary is machine-generated.

A new Vector Control Quantitative Analysis (VCQA) method accurately quantifies multiple species in Traditional Chinese Medicine (TCM) formulas. This technique addresses product mislabeling and quality control issues in complex herbal medicines.

Keywords:
complex herbal formulasinternal transcribed spacer regionlimit of quantificationspecies quantificationtraditional Chinese medicine quality controlvector control quantitative analysis

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Area of Science:

  • Pharmacology and Pharmacognosy
  • Molecular Biology
  • Genetics

Background:

  • Product mislabeling and species fraud in Traditional Chinese Medicine (TCM) compromise quality and pose health risks.
  • Existing quality control methods focus on metabolite detection or species identification, neglecting species quantification in complex formulas.
  • Accurate species quantification is crucial for ensuring the safety and efficacy of TCM formulations.

Purpose of the Study:

  • To develop and validate a novel, flexible, and accurate method for multiplex species quantification in complex TCM herbal formulas.
  • To establish a reliable tool for assessing the quality and authenticity of TCM products.
  • To address the gap in current methods for quantifying individual species within intricate herbal mixtures.

Main Methods:

  • Developed a Polymerase Chain Reaction (PCR)-based strategy for generating integrated DNA fragments from multiple target species.
  • Utilized Golden Gate ligation and Gateway recombination techniques for efficient assembly into a quantitative vector in a single cloning step.
  • Employed the nuclear ribosomal DNA Internal Transcribed Spacer (ITS) region for species quantification.

Main Results:

  • The Vector Control Quantitative Analysis (VCQA) method demonstrated flexibility and accuracy in multiplex species quantification.
  • The method effectively eliminated variations arising from DNA extracts, utilizing universal and species-specific primers.
  • VCQA achieved a limit of quantification as low as 1% for *Asarum sieboldii* Miq. in formula products.
  • Significant variations in the quality stability of ChuanXiong ChaTiao Wan were observed across different manufacturers.

Conclusions:

  • The developed VCQA method offers a powerful and accurate approach for species quantification in complex TCM formulas.
  • VCQA can serve as a valuable alternative method for ensuring the quality and authenticity of TCM products.
  • The findings highlight the need for stringent quality control measures and underscore the potential of molecular methods in TCM standardization.