Related Experiment Video

Updated: Nov 25, 2025

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
07:40

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Published on: May 26, 2023

1.4K

Dan Hong Injection Protects Against Cardiomyocytes Apoptosis by Maintaining Mitochondrial Integrity Through

Ling Zhang1, Yu Wang1, Chang Li1

  • 1College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.

Frontiers in Pharmacology
|December 16, 2020
PubMed

Insights

Danhong injection protects heart cells from injury by preserving mitochondrial function and reducing cell death. This study reveals its mechanism involves the Keap1/Nrf2/JNK pathway, offering a new treatment for myocardial ischemia/reperfusion injury.

Area of Science:

  • Cardiology
  • Mitochondrial Biology
  • Pharmacology

Background:

  • Danhong injection (DHI) is a traditional Chinese medicine used for cardiovascular diseases.
  • Its mitochondria-protective effects in myocardial ischemia/reperfusion (I/R) injury are recognized but not fully understood.
  • Understanding DHI's mechanism is crucial for optimizing its therapeutic application.

Purpose of the Study:

  • To investigate the effect and mechanism of DHI on mitochondrial integrity and cardiomyocyte apoptosis following I/R injury.
  • To elucidate DHI's role in protecting against hypoxia-reoxygenation (H/R) induced cellular damage.
  • To identify the specific molecular pathways involved in DHI's cardioprotective actions.

Main Methods:

  • Established an I/R rat model to assess infarct size, apoptosis, and oxidative stress.
  • Utilized H9C2 cells subjected to H/R, with and without nuclear factor erythroid 2-related factor 2 (Nrf2) knockdown.
  • Evaluated apoptosis rates, reactive oxygen species (ROS) generation, ATP levels, mitochondrial membrane potential, and oxygen consumption.
  • Analyzed the involvement of the Keap1/Nrf2/JNK pathway.

Main Results:

  • DHI significantly reduced infarct size, apoptosis, and oxidative stress in I/R rat hearts.
  • In vitro, DHI inhibited H/R-induced apoptosis, decreased ROS generation, and preserved mitochondrial integrity.
  • DHI maintained mitochondrial morphology, alleviated dysfunction, increased ATP levels, and enhanced oxygen consumption.
  • The Keap1/Nrf2/JNK pathway was identified as a key mediator of DHI's protective effects.

Conclusions:

  • Danhong injection demonstrates significant cardioprotective effects against I/R injury by preserving mitochondrial integrity and inhibiting apoptosis.
  • DHI exerts its protective effects via the Keap1/Nrf2/JNK pathway, modulating oxidative stress and mitochondrial function.
  • These findings reveal a novel mechanism for DHI and position it as a promising mitochondrial protectant for myocardial I/R injury.

Keywords:
apoptosisdanhong injectionmitochondrial functionmitochondrial morphologymyocardial ischemia/reperfusion injury

More Related Videos

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation07:14

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

14.8K
Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation09:53

Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation

Published on: January 10, 2025

678

Related Concept Videos

The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
7.3K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
7.5K
Cardiomyopathy II: Dilated Cardiomyopathy01:30

Cardiomyopathy II: Dilated Cardiomyopathy

Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
193