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Related Experiment Video

Updated: Nov 25, 2025

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

Intestinal Organoids Generated from Human Pluripotent Stem Cells.

Satoru Tsuruta^1,2, Hajime Uchida³, Hidenori Akutsu²

¹Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.

|December 16, 2020

Summary

Human pluripotent stem cells (PSCs) create three-dimensional intestinal organoids in vitro. These organoids model human digestive organs for disease research and drug development, advancing biomedicine.

Keywords:

drug discovery embryonic stem cells gastrointestinal disease induced pluripotent stem cells intestinal organoids

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Last Updated: Nov 25, 2025

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

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Innervation of Human Intestinal Organoids

Innervation of Human Intestinal Organoids

Published on: January 17, 2025

Area of Science:

Gastroenterology and Regenerative Medicine
Stem Cell Biology and Organoid Technology

Background:

The gastrointestinal system's complexity necessitates advanced in vitro models for studying development, homeostasis, and disease.
Human pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), offer significant potential for modeling complex organ systems.
Existing in vitro models are being enhanced by the development of three-dimensional organoids.

Purpose of the Study:

To review gastrointestinal organoid technology derived from PSCs.
To explore the potential applications of these organoids in research and medicine.
To highlight the role of organoids in understanding intestinal development and disease pathogenesis.

Main Methods:

Generation of three-dimensional intestinal organoids from human pluripotent stem cells (PSCs) in vitro.
Characterization of organoids for epithelial and mesenchymal components.
Assessment of recapitulated intestinal functions within the organoid models.

Main Results:

Intestinal organoids derived from PSCs contain both epithelial and mesenchymal tissues.
These organoids partially recapitulate key intestinal functions.
Organoids have demonstrated utility in disease modeling and drug development.

Conclusions:

Gastrointestinal organoid technology represents a significant advancement in studying digestive organ development and function.
PSC-derived organoids are valuable tools for analyzing molecular interactions and disease mechanisms.
This technology holds substantial promise for the synergistic development of biomedicine for human digestive organs.