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Related Experiment Videos

A temperature-compensated ultradian clock explains temperature-dependent quantal cell cycle times.

D Lloyd1, F Kippert

  • 1Department of Microbiology, University College, Cardiff, Wales, UK.

Symposia of the Society for Experimental Biology
|January 1, 1987
PubMed
Summary

Cell division involves a division protein accumulating throughout the cell cycle. Ultradian rhythms, like those in eukaryotes, exhibit temperature-independent oscillations crucial for cell timekeeping.

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Area of Science:

  • Cell Biology
  • Chronobiology
  • Biophysics

Background:

  • Cell division mechanisms have been studied using heat pulses, suggesting a division protein accumulates during the cell cycle.
  • Mammalian cell cycle control may involve limit-cycle oscillators, with applications in cancer hyperthermia.
  • Circadian and ultradian rhythms share unique features, including temperature independence observed in unicellular organisms.

Purpose of the Study:

  • To investigate the molecular basis of cell division and rhythmicity.
  • To explore the characteristics of ultradian rhythms in unicellular eukaryotes.
  • To determine if ultradian rhythms are temperature-compensated and endogenous.

Main Methods:

  • Utilized centrifugal size selection to prepare synchronous cultures of lower eukaryotes.

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  • Compared biochemical parameters (respiration, RNA, protein) in synchronous versus asynchronous control cultures.
  • Assessed the effect of growth temperature on ultradian rhythm periods in Acanthamoeba castellanii.

    • Main Results:

    • Synchronous cultures exhibited oscillatory behavior in respiration, RNA, protein, and enzyme activities, unlike asynchronous controls.
    • Observed distinct ultradian periods (30-70 min) in different species (A. castellanii, T. pyriformis, C. utilis).
    • Ultradian rhythm periods in A. castellanii showed minimal temperature dependence between 20-30°C and persisted for 12 hours without damping.

    Conclusions:

    • Ultradian rhythms in unicellular eukaryotes are temperature-compensated and endogenous, functioning as internal timekeepers.
    • These findings support the concept of a division protein accumulating during the cell cycle.
    • The temperature independence of these rhythms has implications for understanding cellular processes and potentially for therapeutic applications.