Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

7.6K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
7.6K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

10.8K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
10.8K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.2K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.2K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.7K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.7K
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

135
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
135

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Low Matrix Effects Analytical Strategy for Diazepam Analysis in Aquatic Products Through Immunomagnetic Beads Purification.

Foods (Basel, Switzerland)·2026
Same author

Three-Dimensional Reconstruction of Cephalopod Beaks: A 3D Modeling Workflow Combining Prism-Assisted Photogrammetry and Micro-CT Validation.

Ecology and evolution·2026
Same author

VPS34 inhibition as a host-targeting anti-coronaviral strategy: Rational design of YBM with optimized pharmacokinetic parameters.

Acta pharmaceutica Sinica. B·2026
Same author

Co-occurrence network characteristics and key comorbidity node identification based on health examination indicators among rural older adults aged 65 and above.

BMC geriatrics·2026
Same author

Cadmium Enrichment Characteristics in Different <i>Oratosquilla oratoria</i> Tissues During Various Gonadal Development Stages from Shanghai and Its Health Risk Assessment.

Foods (Basel, Switzerland)·2026
Same author

Multi-omics mendelian randomization integrating GWAS and eQTL data revealed potential drug target for irritable bowel syndrome.

Frontiers in genetics·2026

Related Experiment Video

Updated: Nov 25, 2025

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

19.0K

400  MHz ultrafast optical coherence tomography.

Dongmei Huang, Feng Li, Zijian He

    Optics Letters
    |December 16, 2020
    PubMed
    Summary

    Researchers developed a 400 MHz ultrafast swept source, the fastest for optical coherence tomography (OCT). This advancement enables high-speed OCT imaging with improved resolution and signal-to-noise ratio.

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Laser Technology

    Background:

    • Optical Coherence Tomography (OCT) requires high-speed swept sources for advanced imaging.
    • Existing swept sources have limitations in sweep rate, impacting imaging speed and resolution.
    • Ultrafast laser technology offers potential for overcoming these limitations.

    Purpose of the Study:

    • To demonstrate an ultrafast time-stretched swept source with a record-high sweep rate.
    • To achieve high-resolution OCT imaging of dynamic samples.
    • To improve the signal-to-noise ratio in OCT imaging.

    Main Methods:

    • Utilized a 100 MHz femtosecond laser pulse train with buffering to achieve a 400 MHz sweep rate.
    • Developed and applied a composite complex apodization method.

    More Related Videos

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    3.5K
    Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
    08:50

    Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

    Published on: February 9, 2019

    8.0K

    Related Experiment Videos

    Last Updated: Nov 25, 2025

    Doppler Optical Coherence Tomography of Retinal Circulation
    10:46

    Doppler Optical Coherence Tomography of Retinal Circulation

    Published on: September 18, 2012

    19.0K
    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    3.5K
    Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
    08:50

    Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

    Published on: February 9, 2019

    8.0K
  • Performed OCT imaging of high-speed rotating disks.

    • Main Results:

    • Achieved a sweep rate of 400 MHz, the highest reported for OCT swept sources.
    • Obtained an axial resolution of 19 µm with a 10 dB sweep range of ~100 nm.
    • Successfully demonstrated OCT imaging of high-speed rotating objects.

    Conclusions:

    • The demonstrated 400 MHz swept source is a significant advancement for ultrafast OCT.
    • The proposed apodization method effectively enhances OCT image quality.
    • This technology holds promise for high-speed, high-resolution biomedical and industrial imaging applications.