Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Schistosome elastases: biological importance, structure, function and stage-specific expression.

G Newport1, J McKerrow, R Hedstrom

  • 1University of California Naval Biosciences Laboratory Naval Supply Center, Oakland, CA 94625.

Biochemical Society Symposium
|January 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does dental undergraduate education and postgraduate training enable intention to provide inhalation sedation in primary dental care? A path analytical exploration.

European journal of dental education : official journal of the Association for Dental Education in Europe·2016
Same author

Assessing sedation need and managing referred dentally anxious patients: is there a role for the Index of Sedation Need?

British dental journal·2015
Same author

Antigenic variation in African trypanosomes: DNA rearrangements program immune evasion.

Immunology today·2014
Same author

Substrate specificity profiling and identification of a new class of inhibitor for the major protease of the SARS coronavirus.

Biochemistry·2007
Same author

Effect on antibody and T-cell responses of mixing five GMP-produced DNA plasmids and administration with plasmid expressing GM-CSF.

Genes and immunity·2004
Same author

Safety of a GM-CSF adjuvant-plasmid DNA malaria vaccine.

Gene therapy·2001

Schistosome larvae secrete a potent serine protease to penetrate host skin. This enzyme

Area of Science:

  • Parasitology
  • Biochemistry
  • Molecular Biology

Background:

  • Schistosome larvae invade hosts by skin penetration, secreting macromolecules.
  • A dominant serine protease facilitates this invasion process.

Purpose of the Study:

  • To identify and characterize the schistosome serine protease involved in host invasion.
  • To investigate the enzyme's properties, gene expression, and potential applications.

Main Methods:

  • Enzyme characterization (molecular mass, pI, substrate specificity).
  • Amino acid sequencing and oligonucleotide synthesis.
  • Northern analysis and cDNA isolation/sequencing.

Main Results:

  • Identified a 30 kDa serine protease with elastase-like specificity, degrading multiple host matrix proteins.

Related Experiment Videos

  • Confirmed stage-specific, transcriptionally controlled expression of the enzyme.
  • Demonstrated that the enzyme is encoded by a multigene family.

    • Conclusions:

    • The characterized serine protease is a key factor in schistosome host invasion.
    • Understanding this enzyme opens avenues for anti-schistosome vaccines, inhibitors, and diagnostics.