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Related Experiment Video

Updated: Nov 25, 2025

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Published on: May 22, 2020

MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments.

Pedro M Martins^1,2, Susanna Navarro³, Alexandra Silva¹

¹Instituto de Biologia Molecular e Celular and Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.

Frontiers in Molecular Neuroscience

|December 17, 2020

Summary

This summary is machine-generated.

Standardized guidelines called Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) were developed to improve protein aggregation studies. These guidelines enhance reproducibility and data sharing for disease mechanism research.

Keywords:

amyloid peptide phase separation protein reproducible data

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Last Updated: Nov 25, 2025

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Automating Aggregate Quantification in Caenorhabditis elegans

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Published on: October 14, 2021

Area of Science:

Biochemistry
Molecular Biology
Biophysics

Background:

Protein aggregation and phase separation are crucial in understanding human diseases.
Current research lacks standardized methods, hindering reproducibility and data interpretation.
Existing data is valuable for drug screening and developing aggregation prediction algorithms.

Purpose of the Study:

To establish standardized guidelines for reproducible protein aggregation experiments.
To address the need for detailed methods and aggregate descriptions.
To facilitate data sharing and improve the reliability of aggregation studies.

Main Methods:

Developed the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines.
Incorporated first-principles and existing literature on protein self-assembly.
Focused on essential determinants of experimental reproducibility.

Main Results:

The MIRRAGGE guidelines provide a comprehensive framework for experimental design and reporting.
A MIRRAGGE table template is available to guide researchers.
The guidelines aim to improve the consistency and comparability of aggregation assay results.

Conclusions:

Standardized reporting through MIRRAGGE enhances the reproducibility of protein aggregation experiments.
These guidelines facilitate accurate data deposition into databases.
MIRRAGGE supports translational research, drug discovery, and the development of predictive models for aggregation-related diseases.