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MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments
Pedro M Martins1,2, Susanna Navarro3, Alexandra Silva1
1Instituto de Biologia Molecular e Celular and Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
Abstract:
Reports on phase separation and amyloid formation for multiple proteins and aggregation-prone peptides are recurrently used to explore the molecular mechanisms associated with several human diseases. The information conveyed by these reports can be used directly in translational investigation, e.g., for the design of better drug screening strategies, or be compiled in databases for benchmarking novel aggregation-predicting algorithms. Given that minute protocol variations determine different outcomes of protein aggregation assays, there is a strong urge for standardized descriptions of the different types of aggregates and the detailed methods used in their production. In an attempt to address this need, we assembled the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines, considering first-principles and the established literature on protein self-assembly and aggregation. This consensus information aims to cover the major and subtle determinants of experimental reproducibility while avoiding excessive technical details that are of limited practical interest for non-specialized users. The MIRRAGGE table (template available in Supplementary Information) is useful as a guide for the design of new studies and as a checklist during submission of experimental reports for publication. Full disclosure of relevant information also enables other researchers to reproduce results correctly and facilitates systematic data deposition into curated databases.
Insights
Standardized guidelines called Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) were developed to improve protein aggregation studies. These guidelines enhance reproducibility and data sharing for disease mechanism research.
Area of Science:
- Biochemistry
- Molecular Biology
- Biophysics
Background:
- Protein aggregation and phase separation are crucial in understanding human diseases.
- Current research lacks standardized methods, hindering reproducibility and data interpretation.
- Existing data is valuable for drug screening and developing aggregation prediction algorithms.
Purpose of the Study:
- To establish standardized guidelines for reproducible protein aggregation experiments.
- To address the need for detailed methods and aggregate descriptions.
- To facilitate data sharing and improve the reliability of aggregation studies.
Main Methods:
- Developed the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines.
- Incorporated first-principles and existing literature on protein self-assembly.
- Focused on essential determinants of experimental reproducibility.
Main Results:
- The MIRRAGGE guidelines provide a comprehensive framework for experimental design and reporting.
- A MIRRAGGE table template is available to guide researchers.
- The guidelines aim to improve the consistency and comparability of aggregation assay results.
Conclusions:
- Standardized reporting through MIRRAGGE enhances the reproducibility of protein aggregation experiments.
- These guidelines facilitate accurate data deposition into databases.
- MIRRAGGE supports translational research, drug discovery, and the development of predictive models for aggregation-related diseases.
