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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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Related Experiment Video

Updated: Nov 25, 2025

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
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Long Non-coding RNA Expression Profiling Identifies a Four-Long Non-coding RNA Prognostic Signature for Isocitrate

Yusheng Chen1, Yang Guo1, Hang Chen1

  • 1Department of Neurosurgery, Henan Provincial People's Hospital, Zhengzhou, China.

Frontiers in Neurology
|December 17, 2020
PubMed
Summary
This summary is machine-generated.

A new four-long non-coding RNA (lncRNA) signature effectively predicts prognosis in isocitrate dehydrogenase (IDH) mutant glioma. This discovery offers a novel prognostic marker for improving patient outcomes.

Keywords:
IDH mutantgliomalncRNA profileprognosisrisk signature

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Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Isocitrate dehydrogenase (IDH) mutations are key genetic drivers in glioma.
  • The regulatory role of long non-coding RNAs (lncRNAs) in IDH-mutant glioma remains underexplored.

Purpose of the Study:

  • To identify and validate a lncRNA-based signature for prognostic prediction in IDH-mutant glioma.
  • To investigate the biological pathways associated with the identified lncRNA signature.

Main Methods:

  • Analysis of transcriptome data from 775 IDH-mutant glioma samples across multiple datasets (CGGA, TCGA, GSE16011).
  • Identification of differentially expressed lncRNAs between IDH-mutant and IDH-wild-type glioma.
  • Development and validation of a four-lncRNA risk signature (JAG1, PVT1, H19, HAR1A).
  • Statistical analysis using R language and GraphPad Prism, including multivariate Cox regression, Gene Ontology, and KEGG pathway analysis.

Main Results:

  • A four-lncRNA signature comprising JAG1, PVT1, H19, and HAR1A was identified in IDH-mutant glioma.
  • The signature successfully stratified patients into low-risk and high-risk groups across independent cohorts.
  • The four-lncRNA signature demonstrated independent prognostic value, even after adjusting for clinicopathologic factors.
  • Gene Ontology and KEGG analyses linked the signature to immune response and cellular metabolism pathways.

Conclusions:

  • The identified four-lncRNA signature serves as a novel and robust prognostic biomarker for IDH-mutant glioma.
  • This signature has the potential to enhance clinical decision-making and improve outcomes for glioma patients.
  • Further research into the functional roles of these lncRNAs could reveal therapeutic targets.