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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Nov 25, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Efficient Genome Editing in Populus Using CRISPR/Cas12a.

Yi An1, Ya Geng1, Junguang Yao1

  • 1State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, China.

Frontiers in Plant Science
|December 17, 2020
PubMed
Summary
This summary is machine-generated.

The CRISPR/Cas12a system, particularly AsCas12a, efficiently induces targeted mutations and large deletions in poplar trees. This advanced genome editing tool enhances forest tree biotechnology and genetic studies.

Keywords:
CRISPRCas12aPagPDSPopulusgenome editingheat stress

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Area of Science:

  • Plant Biotechnology
  • Genome Editing
  • Forestry

Background:

  • Current CRISPR/Cas9 technology has limitations for forest tree biotechnology.
  • CRISPR/Cas12a offers a broader targeting range and capability for large-fragment deletions.

Purpose of the Study:

  • To evaluate the CRISPR/Cas12a system for targeted mutagenesis in poplar (Populus alba × Populus glandulosa).
  • To compare the efficiency of different Cas12a nucleases (AsCas12a, LbCas12a, FnCas12a).
  • To optimize conditions for enhancing CRISPR/Cas12a editing efficiency in poplar.

Main Methods:

  • Utilized CRISPR/Cas12a genome-targeting system to induce mutations in the PDS gene of poplar.
  • Tested three Cas12a nucleases: AsCas12a, LbCas12a, and FnCas12a.
  • Optimized Agrobacterium-mediated transformation co-cultivation temperature from 22°C to 28°C.

Main Results:

  • AsCas12a demonstrated the highest mutation efficiency (70%) in poplar.
  • The majority of edits induced by AsCas12a resulted in large-fragment deletions.
  • Optimizing co-cultivation temperature to 28°C improved Cas12a nuclease editing efficiency.

Conclusions:

  • The CRISPR/Cas12a system, especially AsCas12a, is highly efficient for targeted mutagenesis in poplar.
  • This system facilitates multigene simultaneous knockout mutants in tree species.
  • CRISPR/Cas12a provides powerful tools for advancing genetic studies in forest trees.