Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Injectable conductive chitosan/dextran/polypyrrole hydrogel for cardiac repair with potential application in radiation-induced cardiac injury.

International journal of biological macromolecules·2026

Same author

Small Organic Molecule-Based Multicolor Fluorescent Smart Materials: From Design Principles and Optical Properties to Versatile Applications.

ACS central science·2026

Same author

Harnessing interfacial click polymerization using pyridinium-yne films as photochromic, radical generation and sensing platforms.

Nature communications·2026

Same author

LlSAPK2 confers thermotolerance in lily by phosphorylating and stabilizing LlbZIP46.

Journal of integrative plant biology·2026

Same author

Synergistic Singlet-Triplet Regulation in Platinum(II)-Acetylide Triads with Strong Two-Photon Absorption and Optical Power Limiting.

The journal of physical chemistry. B·2026

Same author

Interfacial Fluorescent Nanofilms for Rapid and Discriminative Detection of Diethyl Chlorophosphate and Thionyl Chloride.

ACS applied materials & interfaces·2026

Search research articles

Related Experiment Video

Updated: Nov 25, 2025

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

Array-Based Discriminative Optical Biosensors for Identifying Multiple Proteins in Aqueous Solution and Biofluids.

Junmei Fan¹, Lu Qi², Hongfei Han¹

¹Department of Chemistry, Taiyuan Normal University, Jinzhong, China.

Frontiers in Chemistry

|December 17, 2020

Summary

This review overviews discriminative optical biosensors for protein identification, crucial for medical research and disease diagnosis. It categorizes these biosensors to advance their development and applications.

Keywords:

amphiphilic aggregate environment-sensitive system nanoparticles protein sensor array

More Related Videos

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

Related Experiment Videos

Last Updated: Nov 25, 2025

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

Area of Science:

Biomedical Engineering
Analytical Chemistry
Biosensing Technology

Background:

Protein identification is vital for medical research and clinical diagnostics due to its link to various diseases.
Optical sensor arrays offer advanced capabilities for distinguishing similar analytes and analyzing complex samples.

Purpose of the Study:

To provide a comprehensive overview of array-based discriminative optical biosensors for protein recognition.
To discuss recent developments, construction strategies, and detection mechanisms of these biosensors.
To explore their applications in real-world samples and identify future prospects.

Main Methods:

Categorization of biosensors based on system complexity: multi-element, environment-sensitive, and multi-wavelength single systems.
Detailed introduction to the construction of sensing platforms for each category.
Explanation of the detection mechanisms employed by different sensor strategies.

Main Results:

The review categorizes discriminative optical biosensors into three main types based on their design complexity.
It details the sensing platform construction and detection principles for each category.
Differences and interconnections between these strategies are discussed.

Conclusions:

Array-based discriminative optical biosensors are effective tools for protein identification in complex biological samples.
Understanding the different strategies facilitates the development of novel biosensors.
These advancements hold promise for expanding applications in medical research and clinical practice.