Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of ClockΔ19 mutation on neural recording performance and neural inflammatory responses.

Biomaterials·2026
Same author

Tonic dopamine sensing reveals a D2 and D3-mediated dopamine response to raclopride in <i>Clock</i>Δ<i>19</i> mice model.

Npj biosensing·2026
Same author

Editorial: Carbon microelectrodes for neurochemical sensing.

Frontiers in bioengineering and biotechnology·2026
Same author

Zwitterionic polymer coating enabled chronic dopamine sensing and electrophysiology recording in free-moving mice.

bioRxiv : the preprint server for biology·2026
Same author

In vivo microelectrode arrays for neuroscience.

Nature reviews. Methods primers·2026
Same author

The Single-Prolonged Stress Model Fails to Produce Behavioral or Corticosterone Alterations in Rats.

eNeuro·2026

Related Experiment Video

Updated: Nov 25, 2025

Quantifying Infra-slow Dynamics of Spectral Power and Heart Rate in Sleeping Mice
10:56

Quantifying Infra-slow Dynamics of Spectral Power and Heart Rate in Sleeping Mice

Published on: August 2, 2017

10.3K

Real-Time Fast Scan Cyclic Voltammetry Detection and Quantification of Exogenously Administered Melatonin in Mice

Elisa Castagnola1, Elaine M Robbins1,2, Kevin M Woeppel1,3

  • 1Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United States.

Frontiers in Bioengineering and Biotechnology
|December 17, 2020
PubMed
Summary

Researchers developed a new method for real-time brain monitoring of melatonin (MT). Fast scan cyclic voltammetry (FSCV) with pre-activated electrodes enables sensitive and stable detection of MT, crucial for understanding neurological functions.

Keywords:
braincarbon fiber microelectrodeselectrochemical impedance spectroscopyfast scan cyclic voltammetryfoulingmelatonin

More Related Videos

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
08:58

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Published on: June 19, 2019

10.2K
Polygraphic Recording Procedure for Measuring Sleep in Mice
08:45

Polygraphic Recording Procedure for Measuring Sleep in Mice

Published on: January 25, 2016

24.8K

Related Experiment Videos

Last Updated: Nov 25, 2025

Quantifying Infra-slow Dynamics of Spectral Power and Heart Rate in Sleeping Mice
10:56

Quantifying Infra-slow Dynamics of Spectral Power and Heart Rate in Sleeping Mice

Published on: August 2, 2017

10.3K
Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
08:58

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Published on: June 19, 2019

10.2K
Polygraphic Recording Procedure for Measuring Sleep in Mice
08:45

Polygraphic Recording Procedure for Measuring Sleep in Mice

Published on: January 25, 2016

24.8K

Area of Science:

  • Neuroscience
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Melatonin (MT) shows therapeutic potential for sleep disorders and neurological conditions.
  • Accurate, real-time measurement of brain MT levels is essential for research.
  • Previous methods like square wave voltammetry (SWV) have limitations.

Purpose of the Study:

  • To establish a novel method for detecting exogenous melatonin in the brain.
  • To evaluate the performance of fast scan cyclic voltammetry (FSCV) for in vivo MT detection.
  • To assess the stability and sensitivity of electrochemically pre-activated carbon fiber microelectrodes (CFEs).

Main Methods:

  • Utilized fast scan cyclic voltammetry (FSCV) on electrochemically pre-activated carbon fiber microelectrodes (CFEs).
  • Performed in vitro and in vivo assessments of CFE stability and fouling.
  • Validated FSCV results with square wave voltammetry (SWV) and microdialysis.

Main Results:

  • Achieved the highest sensitivity (28.1 nA/μM) and lowest detection limit (20.2 ± 4.8 nM) for MT detection at carbon surfaces.
  • Demonstrated prolonged, stable in vivo background and minimized electrode fouling with CFE pre-conditioning.
  • Enabled drift-free FSCV detection of MT in mouse brain for up to 3 minutes, exceeding typical acquisition times.

Conclusions:

  • Fast scan cyclic voltammetry (FSCV) offers a reliable and sensitive method for detecting exogenous melatonin in the brain.
  • Pre-activated carbon fiber microelectrodes (CFEs) provide stable and consistent performance for long-term in vivo monitoring.
  • This technique has the potential to significantly advance the study of brain functions and melatonin's role.