The Research Advance of Cell Bridges in vitro

The microenvironment in which cells reside in vivo dictates their biological and mechanical functioning is associated with morphogenetic and regenerative processes and may find implications in regenerative medicine and tissue engineering. The development of nano- and micro-fabricated technologies, three-dimensional (3D) printing technique, and biomimetic medical materials have enabled researchers to prepare novel advanced substrates mimicking the in vivo microenvironment. Most of the novel morphologies and behaviors of cells, including contact guidance and cell bridges which are observed in vivo but are not perceived in the traditional two-dimensional (2D) culture system, emerged on those novel substrates. Using cell bridges, cell can span over the surface of substrates to maintain mechanical stability and integrity of tissue, as observed in physiological processes, such as wound healing, regeneration and development. Compared to contact guidance, which has received increased attention and is investigated extensively, studies on cell bridges remain scarce. Therefore, in this mini-review, we have comprehensively summarized and classified different kinds of cell bridges formed on various substrates and highlighted possible biophysical mechanisms underlying cell bridge formation for their possible implication in the fields of tissue engineering and regenerative medicine.