Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alcoholic fermentation by 'non-fermentative' yeasts.

J P van Dijken1, E van den Bosch, J J Hermans

  • 1Department of Microbiology and Enzymology, Delft University of Technology, The Netherlands.

Yeast (Chichester, England)
|June 1, 1986
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic value of radiologic and pathological response in colorectal cancer liver metastases upon systemic induction treatment: subgroup analysis of the CAIRO5 trial.

ESMO open·2024
Same author

Cardiac Function and Serum Biomarkers throughout Staged Fontan Palliation: A Prospective Observational Study.

Journal of cardiovascular development and disease·2023
Same author

Routine contrast-enhanced CT is insufficient for TNM-staging of duodenal adenocarcinoma.

Abdominal radiology (New York)·2022
Same author

Quantitative CT perfusion imaging in patients with pancreatic cancer: a systematic review.

Abdominal radiology (New York)·2021
Same author

Continuity of care experienced by patients in a multi-institutional pancreatic care network: a pilot study.

BMC health services research·2021
Same author

Does perfusion computed tomography correlate to pathology in colorectal liver metastases?

PloS one·2021

The classical Durham tube test for yeast fermentation is unreliable. Many

Area of Science:

  • Microbiology
  • Yeast Taxonomy
  • Biochemistry

Background:

  • The classification of yeasts as fermentative or non-fermentative traditionally relies on gas production in Durham tubes.
  • Previous studies have not fully explored the fermentative capabilities of yeasts classified as non-fermentative under varying conditions.

Purpose of the Study:

  • To re-evaluate the glucose fermentation capacity of 'non-fermentative' yeast type strains.
  • To determine the reliability of the Durham tube test for classifying yeast fermentation.
  • To investigate yeast fermentation under oxygen-limited conditions.

Main Methods:

  • Reinvestigation of type strains from the Centraalbureau voor Schimmelcultures collection.
  • Glucose fermentation assessment using the Durham tube test.

Related Experiment Videos

  • Analysis of ethanol production under oxygen-limited growth conditions in shake-flask cultures.

    • Main Results:

    • Nearly all tested 'non-fermentative' yeast strains produced significant ethanol, despite the absence of visible gas in Durham tubes.
    • Oxygen-limited conditions induced strong alcoholic fermentation in yeasts like Hansenula nonfermentans and Candida silvae.
    • The presence of ethanol indicates fermentation, even without gas detection.

    Conclusions:

    • The Durham tube test, relying solely on gas detection, is an unreliable method for classifying yeasts as fermentative or non-fermentative.
    • Physiological classification of yeasts requires considering ethanol production alongside gas formation.
    • Environmental conditions, such as oxygen availability, significantly influence yeast fermentation pathways.