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Preparation and characterization of various chitin-glucan complexes derived from white button mushroom using a deep
Hireem Kim1, Seulgi Kang1, Ke Li1
1School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi 16419, Republic of Korea.
International Journal of Biological Macromolecules
|December 17, 2020
Summary
Deep eutectic solvents (DESs) offer an eco-friendly method to extract chitin-glucan complexes (CGCs) from mushrooms. This novel approach yields advanced biomaterials with unique porous structures for diverse applications.
Area of Science:
- Biomaterials Science
- Green Chemistry
- Mycology
Background:
- Deep eutectic solvents (DESs) are recognized as environmentally friendly and safe solvent alternatives.
- Chitin-glucan complexes (CGCs) are valuable biopolymers found in fungal cell walls.
- Mushroom biomass presents an underutilized source for biopolymer extraction.
Purpose of the Study:
- To develop a green and efficient method for preparing chitin-glucan complexes (CGCs) from white button mushrooms (Agaricus bisporus) using DESs.
- To characterize and compare the properties of DES-extracted CGCs with those obtained via traditional alkali-insoluble matter (AIM) methods.
- To evaluate the potential of DES-derived CGCs as advanced biomaterials.
Main Methods:
- Extraction of CGCs from Agaricus bisporus using five different DESs combined with ultrasonication.
- Fractionation of CGCs into DES-soluble (DES_S) and DES-insoluble (DES_P) components.
- Characterization of CGCs, including chitin-to-β-glucan ratio, acetylation degree, thermal stability, crystallinity, and surface morphology, compared to AIM.
Main Results:
- Ten distinct CGCs were successfully prepared using DESs, with varying compositions.
- CGCs derived from betaine and urea (BU_S and BU_P) exhibited high yields and low protein/mineral content.
- BU_S and BU_P demonstrated significantly lower crystallinity and higher surface porosity compared to AIM and commercial chitin, indicating unique material properties.
Conclusions:
- DES-based extraction offers a mild, eco-friendly, and effective route for producing diverse CGCs from mushroom biomass.
- The resulting DES-derived CGCs possess distinct structural characteristics, such as reduced crystallinity and enhanced porosity, making them promising candidates for advanced biomaterial applications.
- This methodology highlights the potential of utilizing fungal resources for sustainable biomaterial development.
