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Electrochemistry of catalase at a liquid|liquid micro-interface array.

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Summary

This study shows catalase (CAT) is electroactive at liquid-liquid interfaces for label-free detection. Its electrochemical behavior at the interface-array (ITIES) depends on pH, enabling detection in complex samples like serum.

Keywords:
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Area of Science:

  • Biomolecular electrochemistry
  • Analytical chemistry
  • Biosensing

Background:

  • Catalase (CAT) is a crucial enzyme.
  • Interface between two immiscible electrolyte solutions (ITIES) offers unique electrochemical detection methods.
  • Non-redox detection of biomolecules is advantageous.

Purpose of the Study:

  • Investigate catalase electrochemistry at the ITIES.
  • Develop a label-free detection strategy for CAT.
  • Assess feasibility in complex matrices like serum.

Main Methods:

  • Electrochemical investigation of CAT at a micro-ITIES array.
  • Cyclic voltammetry to study pH-dependent behavior.
  • Adsorptive stripping voltammetry (AdSV) for sensitive detection.

Main Results:

  • Distinct voltammetric response observed when aqueous phase pH < CAT's isoelectric point (pI).
  • No response when pH > pI, indicating charge-dependent interfacial behavior.
  • AdSV enabled sensitive detection of CAT via interfacial accumulation and desorption.

Conclusions:

  • Catalase exhibits electroactivity at the ITIES.
  • This electroactivity is pH-dependent and can be exploited for label-free detection.
  • Detection in pH-adjusted artificial serum is feasible, showing potential for real-world applications.