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Establishment and Quantification of De Novo Lytic Infection by Cell-free Kaposi's Sarcoma-Associated Herpesvirus

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Establishment of a Challenge Model for Sheeppox Virus Infection.

Janika Wolff¹, Sahar Abd El Rahman², Jacqueline King¹

¹Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, Insel Riems, D-17493 Greifswald, Germany.

|December 18, 2020

Summary

This study investigated Sheeppox virus (SPPV) pathogenesis in sheep using Indian and Egyptian isolates. Researchers established a challenge model for SPPV, crucial for future vaccine development against this economically significant disease.

Keywords:

MinION SPPV capripox challenge model field strain nanopore sequencing next-generation sequencing pathogenesis sheeppox

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Area of Science:

Veterinary Virology
Animal Pathology

Background:

Sheeppox virus (SPPV), alongside goatpox virus and lumpy skin disease virus, belongs to the *Capripoxvirus* genus within the *Poxviridae* family.
Capripox viruses are significant notifiable diseases due to their substantial economic impact on livestock and small-scale farmers.

Purpose of the Study:

To investigate the pathogenesis of Indian and Egyptian Sheeppox virus (SPPV) isolates in sheep.
To establish a reliable challenge model for SPPV infections suitable for future vaccine efficacy studies.

Main Methods:

Infection of sheep via intravenous, intranasal routes, and contact transmission.
Monitoring of clinical signs, viremia, viral shedding, and seroconversion.
Whole-genome sequencing (next- and third-generation) of SPPV isolates for genetic characterization.

Main Results:

Detailed characterization of clinical course, viremia, viral shedding, and seroconversion across different infection routes.
Successful establishment of a challenge model for SPPV.
Generation of high-quality full-length genomes for genetic comparison of SPPV strains.

Conclusions:

The study provides a comprehensive understanding of SPPV pathogenesis in sheep through various infection routes.
The established challenge model is vital for advancing research and development of SPPV vaccines.
Genomic data will aid in understanding SPPV evolution and strain diversity.