Immunestimulation by 4-imino-1,3-diazabicyclo(3.1.0.)-hexan-2-on (BM 06.002)

The new cancerostatic substance 4-imino-1,3-diazabicycle- (3.1.0.)-hexan-2-on was investigated in DBF1 mice in respect to its effects on antibody producing B-lymphocytes and T-lymphocytes. It was shown that BM 06.002 has no definitive influence on immunocompetence when used at a low concentration (1 mg/kg). At higher concentrations of 5 up to 20 mg/kg a stimulation of T-lymphocytes was found which was constant at a concentration of 20 mg/kg for more than three weeks after the single injection of 20 mg/kg. Using the haemolytic plaque test, a stimulation of the antibody producing B-cells was observed on day 3 and 12 after treatment. On day 5 a transitory suppression of the lymphocyte functions was observed in both test systems.