Role of Estrogen Receptor-Mediated Anti-Tumor Effects in U2OS Cells

This study aimed to investigate the effects of 17β-estradiol and estrogen receptors (ERs) in U2OS cells.

Osteosarcoma U2OS cells were divided into six groups, and cell proliferation was determined using the cell counting kit-8 growth test. Furthermore, U2OS cell migration and invasion were examined by cell scratch test and Transwell invasion assays, respectively.

At 48 h of 17β-estradiol exposure, U2OS cell viability decreased (p<0.001); however, ERα siRNA and ERβ siR-NAs significantly increased cell viability (p<0.01). Considering the cell positions at 0 h, the cell migration distance at 24 h significantly reduced in the presence of 17β-estradiol (p<0.001); however, ERα and ERβ siRNAs significantly increased cell migration distance (p<0.01). The number of invasive cells significantly decreased upon exposure to 17β-estradiol (p<0.001); however, ERα and ERβ siRNAs significantly increased the number of invasive cells (p<0.01 and p<0.05, respectively).

17β-estradiol exhibited significant anti-tumor effects on U2OS cells that were mediated by ERs and reduced cell proliferation, migration, and invasion.