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Technical Note: Comparative Study of Three Different Fecal Calprotectin Immunoassays: TriCAL Study
Francisco J Mérida1, Nieves Bel2, Soledad Sánchez-Montes3
1Laboratory Medicine Department Serranía de Ronda Hospital, Ronda franciscoj.merida.sspa@juntadeandalucia.es.
Objective:
Calprotectin is a biomarker of gastrointestinal inflammation and disease activity. We aimed to evaluate the performance of a new fecal calprotectin (FC) test (CALiaGold®) in comparison with two other rapid FC immunoassays.
Methods:
Fecal samples were analyzed with all three FC tests. Correlation between the FC tests was assessed. Agreement to discriminate FC-positive samples was also evaluated according to the manufacturers' cut-off values.
Results:
A correlation coefficient of over 0.800 was found in all comparisons. Lower deviation of linearity was observed between immunoturbidimetric assays. Agreement on FC-positive sample detection was reduced in comparisons with the immunochromatographic assay.
Conclusions:
The CALiaGold® test showed good performance for FC assessment. Better correlation and agreement between immunoturbidimetric assays were confirmed.
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