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Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
Published on: December 11, 2020
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Eugenol protects cells against oxidative stress via Nrf2
Leina Ma1,2, Jia Liu2, Qian Lin1
1Key Laboratory of Marine Drugs (Ministry of Education), Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao, Shandong 266003, P.R. China.
Experimental and Therapeutic Medicine
|December 18, 2020
Summary
Eugenol protects cells against oxidative stress by activating the Nrf2 pathway. This natural compound enhances cellular defense mechanisms, making it a promising candidate for antioxidant development.
Area of Science:
- Biochemistry
- Cell Biology
- Pharmacology
Background:
- Eugenol is a natural compound with known bioactivities.
- Its role in cellular protection against oxidative stress is not well understood.
- Oxidative stress is implicated in various cellular damages.
Purpose of the Study:
- To investigate the effects of eugenol on hydrogen peroxide (H2O2)-induced cellular damage.
- To elucidate the molecular mechanisms underlying eugenol's protective effects.
- To explore eugenol's potential as an antioxidant.
Main Methods:
- Utilized HEK-293 and NIH-3T3 cell lines.
- Assessed the impact of eugenol on oxidative stress markers.
- Measured the transcriptional activity and expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its target genes.
- Investigated Nrf2 stabilization and nuclear translocation.
Main Results:
- Eugenol dose-dependently increased Nrf2 transcriptional activity and expression.
- Upregulation of Nrf2 target genes, including glutamate-cysteine ligase modifier regulatory subunit and glutathione S-transferase A1, was observed.
- Eugenol enhanced Nrf2 stabilization and nuclear translocation.
- Reduced intracellular reactive oxygen species (ROS) levels and increased resistance to H2O2-induced damage were noted, dependent on Nrf2.
Conclusions:
- Eugenol acts as a protective agent against oxidative stress.
- Its protective effects are mediated through a Nrf2-dependent pathway.
- Eugenol and its derivatives show promise for the development of novel antioxidants.
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