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The lncRNA UBE2R2-AS1 suppresses cervical cancer cell growth in vitro

Chunyan Liu1, Huajun Li1, Qinan Yin2

  • 1Department of Obstetrics and Gynaecology, China-Japan Friendship Hospital, Beijing, 100029, China.

Open Medicine (Warsaw, Poland)
|December 18, 2020
PubMed
Abstract

Insights

The long noncoding RNA UBE2R2-AS1 acts as a tumor suppressor in cervical cancer. Its reduced expression in tumors correlates with advanced stages, and restoring it inhibits cancer cell growth and invasion.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cervical cancer remains a significant global health challenge.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific function of lncRNA UBE2R2-AS1 in cervical cancer is not well understood.

Purpose of the Study:

  • To investigate the role and underlying mechanisms of lncRNA UBE2R2-AS1 in cervical cancer progression.
  • To determine the expression levels of UBE2R2-AS1 in cervical cancer tissues and cell lines.
  • To evaluate the impact of UBE2R2-AS1 on cervical cancer cell proliferation, apoptosis, and invasion.

Main Methods:

  • Analysis of UBE2R2-AS1 expression in 34 pairs of cervical cancer and adjacent normal tissues using in situ hybridization.
  • Cellular assays including CCK8, flow cytometry, Transwell, and wound healing to assess proliferation, apoptosis, and invasion.
  • Western blot analysis to measure protein expression levels of apoptosis-related (caspase-3, caspase-8) and invasion-related (MMP-2, MMP-9) markers.

Main Results:

  • UBE2R2-AS1 expression was significantly lower in cervical cancer tissues compared to normal tissues, inversely correlated with cancer stage.
  • Overexpression of UBE2R2-AS1 in HeLa and SiHa cells suppressed cell proliferation and invasion while promoting apoptosis.
  • UBE2R2-AS1 overexpression led to increased levels of caspase-3 and caspase-8, and decreased levels of MMP-2 and MMP-9.

Conclusions:

  • UBE2R2-AS1 functions as a suppressor of cervical cancer cell biological activities.
  • UBE2R2-AS1 exhibits potential as an antitumor factor for cervical cancer therapy.
Keywords:
HeLaSiHaUBE2R2-AS1biological activitiescervical cancer

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