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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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Developments in tandem ion mobility mass spectrometry.

Charles Eldrid1, Konstantinos Thalassinos1,2

  • 1Institute of Structural and Molecular Biology, UCL, Gower St, London WC1E 6BT, U.K.

Biochemical Society Transactions
|December 18, 2020
PubMed
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Ion mobility-mass spectrometry (IM-MS) separates molecules by mass, charge, and shape. Recent advances in tandem IM-MS offer powerful new analytical capabilities for complex biological systems.

Keywords:
ion mobilitymass spectrometryprotein structuretandem ion mobility

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Physical Chemistry

Background:

  • Ion mobility-mass spectrometry (IM-MS) separates ions based on mass-to-charge ratio (m/z), and their size and shape in the gas phase.
  • Tandem mass spectrometry (MS/MS) is a widely used technique for molecular structure elucidation.
  • Instruments capable of multiple rounds of ion mobility selection (tandem IM) are less common but offer enhanced separation power.

Observation:

  • The first commercial IM-MS instrument enabling multiple IM selection rounds was introduced in 2019.
  • Tandem IM-MS instruments combine the separation capabilities of ion mobility with the structural information from mass spectrometry.
  • This combination allows for the separation of complex mixtures and the characterization of specific molecular species.

Findings:

  • This review explores the historical development of tandem IM instrumentation.
  • Recent technological advancements have expanded the capabilities and accessibility of tandem IM-MS.
  • Applications in biological systems, such as proteomics and metabolomics, are increasingly being reported.

Implications:

  • Tandem IM-MS provides a powerful tool for analyzing complex biological samples with high specificity.
  • Future developments are expected to further enhance the resolution and throughput of these instruments.
  • This technique holds significant promise for advancing our understanding of biological processes and disease mechanisms.