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Long non-coding RNA PLAC2 suppresses the survival of gastric cancer cells through down-regulating C-Myc

C-C Wu1, Y Yang, F-F Mao

  • 1Department of Gastrointestinal Surgery, Xinghua People's Hospital, The Affiliated Hospital of Yangzhou University, Taizhou, China. weixingg88@126.com.

European Review for Medical and Pharmacological Sciences
|December 18, 2020
PubMed
Abstract

Insights

Long non-coding RNA PLAC2 (lncRNA PLAC2) suppresses gastric cancer cell progression by downregulating c-Myc. This study reveals lncRNA PLAC2 as a potential therapeutic target for gastric cancer.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Gastric cancer (GC) is a significant global health concern.
  • Long non-coding RNAs (lncRNAs) play crucial roles in various cancers, including GC.
  • The specific role of lncRNA placenta-specific protein 2 (PLAC2) in GC remains to be fully elucidated.

Purpose of the Study:

  • To investigate the functional impact of lncRNA PLAC2 on the biological behaviors of gastric cancer cells.
  • To explore the underlying mechanism involving the regulation of the c-Myc gene by lncRNA PLAC2.

Main Methods:

  • Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) to assess PLAC2 expression in GC tissues and cell lines.
  • Flow cytometry, wound healing, and Transwell assays to evaluate the effects of PLAC2 on GC cell apoptosis, cell cycle, migration, and invasion.
  • Western blotting to determine c-Myc protein levels after PLAC2 manipulation.

Main Results:

  • PLAC2 expression was significantly downregulated in GC tissues and cell lines.
  • Overexpression of PLAC2 led to G1/G0 phase arrest and increased apoptosis in GC cells.
  • Inhibition of GC cell migration and invasion was observed upon PLAC2 overexpression.
  • Both mRNA and protein levels of c-Myc were reduced following PLAC2 overexpression.

Conclusions:

  • LncRNA PLAC2 exerts tumor-suppressive effects on gastric cancer cells.
  • The mechanism involves the downregulation of c-Myc expression.
  • LncRNA PLAC2 represents a potential therapeutic target for gastric cancer treatment.

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