CircRNA_100395 protects breast carcinoma deterioration by targeting MAPK6

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer.
• The specific function of circRNA_100395 in breast carcinoma remains largely unexplored.

Purpose of the Study:

• To investigate the differential expression of circRNA_100395 in breast carcinoma.
• To explore the regulatory effects of circRNA_100395 on breast carcinoma cell phenotypes and its underlying molecular mechanism.

Main Methods:

• Quantitative real-time PCR was used to detect circRNA_100395 expression.
• Cell proliferation, migration, and invasion were assessed using CCK-8, colony formation, and Transwell assays.
• Dual-luciferase reporter and rescue assays were employed to confirm the interaction between circRNA_100395 and MAPK6.

Main Results:

• CircRNA_100395 expression was significantly downregulated in breast carcinoma tissues and cell lines.
• Lower circRNA_100395 levels correlated negatively with advanced tumor staging and larger tumor size.
• Overexpression of circRNA_100395 suppressed breast carcinoma cell proliferation and migration, mediated through the targeting of MAPK6.

Conclusions:

• CircRNA_100395 functions as an anti-cancer gene in breast carcinoma progression by inhibiting MAPK6.
• CircRNA_100395 holds promise as a potential diagnostic biomarker and therapeutic target for breast carcinoma.