Related Experiment Video

Updated: Nov 25, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

5.8K

Linc00475 promotes the progression of glioma by regulating the miR-141-3p/YAP1 axis

Mingjun Yu1,2,3, Bolong Yi1,3, Wen Zhou4

  • 1Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, China.

Journal of Cellular and Molecular Medicine
|December 18, 2020
PubMed

Insights

This study reveals that the long non-coding RNA, linc00475, is elevated in glioma and promotes tumor progression. Inhibiting linc00475 offers a potential therapeutic strategy for glioma by targeting the miR-141-3p/YAP1 pathway.

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Genetics

Background:

  • Glioma is a primary brain tumor with high mortality.
  • Aberrant long non-coding RNAs (lncRNAs) are implicated in glioma oncogenesis.
  • The specific role of linc00475 in glioma remains unclear.

Purpose of the Study:

  • To investigate the function and mechanism of linc00475 in glioma.
  • To determine if linc00475 could serve as a prognostic biomarker or therapeutic target for glioma.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to analyze linc00475 expression.
  • In vitro and in vivo experiments to assess the effect of linc00475 inhibition on glioma progression.
  • Luciferase reporter assays to confirm the interaction between linc00475, miR-141-3p, and YAP1.
  • Rescue assays to validate the regulatory pathway.

Main Results:

  • linc00475 expression is significantly upregulated in glioma tissues compared to normal brain tissues.
  • High linc00475 levels correlate with poor prognosis in glioma patients.
  • Inhibition of linc00475 suppressed glioma cell proliferation and tumor growth in vivo.
  • linc00475 acts as a molecular sponge for miR-141-3p, thereby upregulating YAP1 expression.
  • The miR-141-3p/YAP1 pathway mediates the oncogenic effects of linc00475 in glioma.

Conclusions:

  • linc00475 plays a critical role in glioma development and progression.
  • linc00475 functions via the miR-141-3p/YAP1 axis.
  • linc00475 represents a potential diagnostic biomarker and therapeutic target for glioma.
Keywords:
YAP1ceRNAgliomalinc00475miR-141-3pprognosis

More Related Videos

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.2K
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

11.3K

Related Concept Videos

Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.3K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.1K