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Improved HPLC determination of urinary neopterin
J D Dewitte1, F Berthou, Y Dreano
1Laboratoire de Biochimie: Unité de Chromatographie, Faculté de Médecine de Brest, France.
Biomedical Chromatography : BMC
|January 1, 1987
Summary
This study optimized high-performance liquid chromatography (HPLC) for measuring urinary neopterin, a biomarker useful for diagnosing lung diseases and monitoring kidney transplant rejection.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Biochemistry
Background:
- Urinary neopterin measurement is crucial for diagnosing various diseases.
- Existing methods for neopterin analysis require optimization for routine clinical use.
Purpose of the Study:
- To reevaluate and improve the reversed-phase high-performance liquid chromatography (HPLC) method for urinary neopterin measurement.
- To develop a method suitable for automation in routine clinical laboratory settings.
Main Methods:
- Studied chromatographic behavior of 12 pteridines by varying buffer nature, pH, ionic strength, and organic modifier.
- Developed an isocratic HPLC method balancing specificity and analysis time.
Main Results:
- The optimized isocratic HPLC method provides good specificity and reduced analysis time.
- Urinary neopterin levels, normalized to creatinine, were determined in patients with lung diseases and kidney allografts.
- The method demonstrated utility in diagnosing lung neoplasms, sarcoidosis, bronchial asthma, and monitoring kidney transplant rejection.
Conclusions:
- The enhanced HPLC method is well-suited for routine clinical laboratory automation.
- This improved method aids in the diagnosis of lung diseases and the monitoring of kidney transplant rejection episodes.