Estimation of MU for Glucose in Human Serum Using Bottom-up Approach

Glucose is an important material in human metabolism. The establishment of glucose reference measurement procedures and to study the uncertainties of measurement is of great significance. Linear fitting and dilution of reference material were used in the measurement of glucose concentration and they are common operations in daily work. Investigation of the measurement uncertainty of these operations will be of important significance to clinical laboratory medicine. However, in the field of laboratory medicine, related research was rarely reported.

The spectrometric quantification of glucose is an application of the enzymatic reference method with hexokinase. The sources of uncertainty in the measurement process were analyzed. The measurement uncertainties in the study were evaluated according to GUF method and the method introduced by Quantifying Uncertainty in Analytical Measurement (QUAM) was also applied in the evaluation of the measurement of linear fitting.

The standard curve was built successfully according to the measurement procedure recommended by the CDC and the linear equation was y = 0.000807x + 0.001213 (R2 = 0.999179). The measurement uncertainty of glucose in the sample was 0.450,408 mmol/L.

The method for the determination of serum glucose concentration by hexokinase in our laboratory has been successfully established. The measurement uncertainty was consistent with the GUF method and the method introduced by Quantifying Uncertainty in Analytical Measurement (QUAM) in the process of linear fitting when the glucose concentration was measured by the reference method (hexokinase method).