Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Leaky Scanning02:28

Leaky Scanning

5.4K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Brain endothelial cells orchestrate a neuroprotective antiviral state in the CNS in response to peripheral viral pattern sensing.

Immunity·2026
Same author

A designed overlapping variant immunogen pool elicits broad sarbecovirus neutralization.

bioRxiv : the preprint server for biology·2026
Same author

Hepatitis C virus infection dynamics, treatment, and lipid nanoparticle-mediated infection in humanized liver chimeric mouse models.

Science advances·2026
Same author

Hepatitis B virus covalently closed circular DNA formation in murine hepatic cells uncovers a late entry block.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Autoantibodies neutralizing type I IFNs in 40% of patients with WNV encephalitis in seven new cohorts.

Journal of human immunity·2026
Same author

Factor VIII originates primarily from anatomically distinct subsets of liver sinusoidal endothelial cells.

Blood advances·2026

Related Experiment Video

Updated: Nov 25, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

21.7K

TMEM41B Is a Pan-flavivirus Host Factor.

H-Heinrich Hoffmann1, William M Schneider1, Kathryn Rozen-Gagnon1

  • 1Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY, USA.

Cell
|December 18, 2020
PubMed
Summary
This summary is machine-generated.

Scientists identified TMEM41B as a crucial host factor for flavivirus replication. This protein is essential for viral RNA replication, and genetic variations in it can reduce infection rates.

Keywords:
CRISPRTMEM41BVMP1autophagycoronavirusflavivirusflavivirus replication complexmembrane remodeling

More Related Videos

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

9.9K
Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

11.3K

Related Experiment Videos

Last Updated: Nov 25, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

21.7K
Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

9.9K
Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

11.3K

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Flaviviruses are a significant global health concern, causing outbreaks via mosquito and tick bites.
  • Identifying host factors is critical for understanding and controlling viral infections.

Purpose of the Study:

  • To identify host factors essential for flavivirus replication.
  • To characterize the role of TMEM41B in the viral life cycle.

Main Methods:

  • Genome-wide CRISPR-Cas9 loss-of-function screens were employed.
  • Mechanistic studies were conducted to elucidate the function of TMEM41B.

Main Results:

  • TMEM41B is required by all tested members of the Flaviviridae family and also by SARS-CoV-2.
  • Single nucleotide polymorphisms in TMEM41B are associated with reduced flavivirus infection in East Asian populations.
  • TMEM41B facilitates membrane curvature in viral RNA replication complexes.

Conclusions:

  • TMEM41B is a critical host factor for replication across multiple virus families, including Flaviviridae and Coronaviridae.
  • TMEM41B's role in membrane curvature is key to creating a protected environment for viral genome replication.
  • Genetic variations in TMEM41B have implications for host susceptibility to flavivirus infections.