Related Experiment Video
Updated: Nov 25, 2025

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
Published on: January 21, 2015
3D infrared thermospectroscopic imaging
A Aouali1,2, S Chevalier1, A Sommier2
1Arts et Métiers Institute of Technology, Université de Bordeaux, CNRS, INRA, INP, I2M, HESAM, Esplanade des Arts et Métiers, 33400, Talence, France.
This study introduces 3D Infrared Thermospectroscopic Imaging (3DITI), a novel multispectral tomography technique. 3DITI reconstructs 3D thermo-chemical information from complex semi-transparent media, enabling detailed material analysis.
Area of Science:
- Spectroscopy
- Tomography
- Infrared Imaging
Background:
- Multispectral imaging provides detailed spectral information.
- Tomography reconstructs 3D structures from 2D projections.
- Infrared thermography offers non-contact temperature and chemical composition analysis.
Purpose of the Study:
- To develop and validate a 3D multispectral tomography technique in the middle wavelength infrared (MWIR) range.
- To reconstruct 3D spatial cubes of absorptivity and proper emission from semi-transparent materials.
- To demonstrate the capability of the technique for rapid, high-resolution 3D thermo-chemical imaging.
Main Methods:
- Utilized a middle wavelength infrared (MWIR) camera (1.5–5.5 μm) and a multispectral IR monochromator (400 nm–20 μm).
- Employed a sample on a rotary stage to acquire transmittance projections at multiple angular positions.
- Reconstructed 3D absorptivity and emission cubes using a back-projection method based on inverse Radon transform.
Main Results:
- Successfully performed rapid IR absorptivity tomography of a metallic screw in under 1 minute.
- Obtained 3D absorptivity and emission tomographies of a burner flame and microfluidic PFA tubing with water and ethanol.
- Demonstrated the ability to capture detailed 3D thermo-chemical information in complex semi-transparent media.
Conclusions:
- The 3D Infrared Thermospectroscopic Imaging (3DITI) method effectively provides 3D thermo-chemical data for complex semi-transparent media.
- The technique enables rapid, high-resolution 3D reconstruction of material properties.
- The method generates large datasets, presenting challenges in data acquisition, storage, and processing.
Related Concept Videos
Infrared (IR) Spectroscopy: Overview
Different compounds display unique properties due to their...
IR Spectrometers
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview
The ATR process begins by directing a beam...
IR Spectroscopy: Molecular Vibration Overview
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
Applications of IR Spectroscopy: Overview
IR Spectrum
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0%...

