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Related Experiment Video

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A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
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Stability of hepatitis E virus at high hydrostatic pressure processing.

R Johne1, A Wolff1, A K Gadicherla1

  • 1German Federal Institute for Risk Assessment, Max-Dohrn-Straße 8-10, 10589 Berlin, Germany.

International Journal of Food Microbiology
|December 19, 2020
PubMed
Summary
This summary is machine-generated.

High hydrostatic pressure processing (HPP) can reduce Hepatitis E virus (HEV) infectivity in food. Significant HEV inactivation requires high pressure and extended treatment times, as the virus shows relative stability against HPP.

Keywords:
Cell cultureHepatitis E virusHigh hydrostatic pressureInactivation

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Area of Science:

  • Food safety
  • Virology
  • Food processing technology

Background:

  • Hepatitis E virus (HEV) causes acute and chronic hepatitis.
  • Zoonotic HEV genotype 3 is prevalent in Europe, primarily transmitted through contaminated meat products.
  • High hydrostatic pressure processing (HPP) is a nonthermal technology for pathogen inactivation in food.

Purpose of the Study:

  • To evaluate the efficacy of HPP in inactivating HEV genotype 3.
  • To determine the impact of pressure, temperature, and time on HEV infectivity reduction.
  • To develop predictive models for HEV inactivation by HPP.

Main Methods:

  • Cell culture-adapted HEV genotype 3 preparations were subjected to HPP at varying pressures (100-600 MPa) and temperatures (4°C and 20°C).
  • Viral infectivity was quantified by counting fluorescent foci.
  • Transmission electron microscopy was used to examine viral particle integrity post-treatment.
  • Time-course experiments were conducted to assess inactivation kinetics.

Main Results:

  • HPP application led to a gradual decrease in HEV infectivity with increasing pressure and duration.
  • At 400 MPa for 2 minutes, infectivity reductions of 1 log10 (20°C) and 2 log10 (4°C) were observed.
  • Near-complete inactivation (>3.5 log10 reduction) was achieved at 600 MPa, though low levels of infectious virus persisted in some replicates.
  • HEV particles appeared disassembled and distorted at 600 MPa.
  • Time-course experiments at 400 MPa showed a continuous decline in infectivity over 10 minutes.

Conclusions:

  • HPP is effective in reducing HEV infectivity, with inactivation dependent on pressure intensity and treatment duration.
  • HEV demonstrates relative stability against HPP compared to other viruses.
  • Achieving significant HEV inactivation necessitates high pressure and/or extended processing times.