[Determination of the glucoamylase activity in amylolytic preparations]

Glucoamylase capacity of amylolytic preparations was determined with respect to the initial rate (VO) of the glucose synthesis from starch when treated with the preparations tested. The concentration of free glucose (y) was assayed, using a glucooxidase reagent after 5, 10 and 20 min hydrolysis. This method takes into consideration the y -- t function which may be non-linear due to the effect of a number of factors (occurrence of alpha-amylase, enzyme inactivation, etc.). In the case of the y -- t linear function VO is measured on the basis of the angle of the y(t) curve. In the case of the y -- t nonlinear function VO is determined by extrapolation according to the described method. The paper contains examples of how to determine can calculate glucoamylase capacity for the two cases.