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Hydrogenotrophic Methanogenesis Under Alkaline Conditions.

Richard M Wormald1, Simon P Rout1, William Mayes2

  • 1Department of Biological and Geographical Sciences, University of Huddersfield, Huddersfield, United Kingdom.

Frontiers in Microbiology
|December 21, 2020
PubMed
Summary
This summary is machine-generated.

In cement-based geological disposal facilities, hydrogenotrophic methanogenesis is the primary methane generation pathway under alkaline conditions. Acetoclastic methanogenesis is inhibited above pH 9.0 due to substrate accessibility issues.

Keywords:
acetoclastic methanogensalkalinealkaliphileshydrogenotrophic methanogensradioactive waste

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Area of Science:

  • Geochemistry
  • Microbiology
  • Environmental Science

Background:

  • Cement-based geological disposal facilities (GDFs) are considered for intermediate-level radioactive waste.
  • GDFs contain organic and metallic materials, enabling methanogenesis.
  • Cementitious materials create an alkaline environment requiring microbial adaptation.

Purpose of the Study:

  • Determine the upper pH limit for methanogenesis.
  • Identify the dominant methane generation pathway in alkaline environments.
  • Investigate microbial adaptation to alkaline conditions in GDFs.

Main Methods:

  • Investigated alkaline and neutral pH sediments.
  • Assessed methanogenesis routes (acetoclastic vs. hydrogenotrophic).
  • Utilized fluoromethane inhibition studies to confirm syntrophic acetate oxidation.

Main Results:

  • Acetoclastic methanogenesis ceased above pH 9.0.
  • Hydrogenotrophic methanogenesis dominated under alkaline conditions (>pH 9.0).
  • Syntrophic acetate oxidation coupled hydrogenotrophic methanogenesis in some alkaline sediments.

Conclusions:

  • Hydrogenotrophic methanogenesis is the prevalent pathway in alkaline GDF environments.
  • Acetoclastic methanogenesis is limited by substrate availability at high pH.
  • Microbial communities adapt to alkaline conditions, favoring specific metabolic pathways.