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Updated: Nov 25, 2025

Microfluidic Chip Fabrication and Method to Detect Influenza
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Microfluidic-based approaches for COVID-19 diagnosis.

Hsuan-Yu Mu1, Yu-Lun Lu1, Tzu-Hung Hsiao

  • 1Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.

Biomicrofluidics
|December 21, 2020
PubMed
Summary

Rapid diagnostic methods are crucial for controlling the COVID-19 pandemic. This review covers microfluidic platforms for quick virus detection, including qPCR, ELISA, and CRISPR-based approaches for SARS, MERS, and COVID-19.

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Area of Science:

  • * Virology and Molecular Diagnostics
  • * Biomedical Engineering and Microfluidics

Background:

  • * The COVID-19 pandemic, caused by SARS-CoV-2, has led to millions of deaths globally.
  • * Accurate and rapid diagnostic methods are essential for pandemic control and treatment.
  • * Existing diagnostic tools require optimization for speed, accuracy, and accessibility.

Purpose of the Study:

  • * To review current off-site and on-site virus detection methods.
  • * To highlight the integration of diagnostic techniques on microfluidic platforms.
  • * To summarize diagnostic approaches for SARS, MERS, and COVID-19.

Main Methods:

  • * Review of literature on various diagnostic techniques including qPCR, ELISA, and CRISPR-based assays.
  • * Analysis of microfluidic platform implementations for rapid virus screening.
  • * Examination of diagnostic methods applicable to SARS, MERS, and potential COVID-19 detection.

Main Results:

  • * Multiple diagnostic methods, including qPCR, ELISA, and CRISPR, have been successfully adapted for microfluidic platforms.
  • * These integrated systems offer rapid screening capabilities for viral pathogens.
  • * Several methods show potential for detecting SARS, MERS, and COVID-19, with some requiring modifications.

Conclusions:

  • * Microfluidic-based detection offers a promising avenue for developing advanced clinical diagnostic techniques.
  • * These approaches can accelerate examination processes and increase diagnostic accuracy.
  • * Further development can lead to reduced healthcare costs and improved pandemic response.