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Published on: November 5, 2013
An ESIPT-induced NIR fluorescent probe to visualize mitochondrial sulfur dioxide during oxidative stress in vivo
Haixian Ren1, Fangjun Huo2, Xia Wu3
1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. yincx@sxu.edu.cn and Xinzhou Teachers University, Xinzhou 030004, China.
Abstract:
Based on the change in electron distribution of the benzopyrylium unit before and after sulfite addition, a 2-(2'-hydroxyphenyl)benzothiazole (HBT)-based fluorophore generated the excited state intramolecular proton transfer (ESIPT) process with a near-infrared enhanced emission at 836 nm and a large Stokes shift (286 nm). The probe was applied to image SO2 derivatives in cells and mice. Our data will provide new ideas for the development of ESIPT-based fluorescence probe with longer wavelength emission.
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