Temporal gene expression profiling of maslinic acid-treated Raji cells
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Published on: October 4, 2013
Wai Meng Lau1, Menaga Subramaniam1, Hoe Han Goh2
1Centre for Cancer Research, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, PT21144, Jalan Sungai Long, Bandar Sungai Long, 43000 Kajang, Selangor, Malaysia. ymlim@utar.edu.my.
Abstract:
Maslinic acid is a novel phytochemical reported to target multiple signaling pathways. A complete gene expression profile was therefore constructed to illustrate the anti-tumourigenesis effects of maslinic acid in Raji cells across five time-points. Microarray analysis was used to identify genes that were differentially expressed in maslinic acid treated Raji cells at 0, 4, 8, 12, 24 and 48 h. Extracted RNA was hybridized using the AffymetrixGeneChip to obtain expression profiles. A total of 109 genes were found to be significantly expressed over a period of 48 hours. By 12 hours, maslinic acid regulates the majority of genes involved in the cell cycle, p53 and NF-κB signaling pathways. At the same time, XAF1, APAF1, SESN3, and TP53BP2 were evidently up-regulated, while oncogenes, FAIM, CD27, and RRM2B, were down-regulated by at least 2-fold. In conclusion, maslinic acid shows an hourly progression of gene expression in Raji cells.
Insights
Maslinic acid, a phytochemical, impacts anti-tumorigenesis by altering gene expression in Raji cells. It regulates key pathways like cell cycle, p53, and NF-κB over 48 hours.
Area of Science:
- * Molecular Biology
- * Cancer Research
- * Phytochemistry
Background:
- * Maslinic acid is a novel phytochemical.
- * It targets multiple signaling pathways.
- * Its anti-tumorigenesis effects require detailed investigation.
Purpose of the Study:
- * To construct a complete gene expression profile of maslinic acid in Raji cells.
- * To illustrate the anti-tumourigenesis effects of maslinic acid.
- * To analyze gene expression changes across five time-points (0, 4, 8, 12, 24, and 48 hours).
Main Methods:
- * Gene expression profiling using microarray analysis.
- * Hybridization of extracted RNA using AffymetrixGeneChip.
- * Identification of differentially expressed genes in maslinic acid-treated Raji cells.
Main Results:
- * A total of 109 genes were significantly expressed over 48 hours.
- * Maslinic acid regulated cell cycle, p53, and NF-κB signaling pathways by 12 hours.
- * Upregulation of XAF1, APAF1, SESN3, TP53BP2 and downregulation of oncogenes FAIM, CD27, RRM2B observed.
Conclusions:
- * Maslinic acid exhibits an hourly progression of gene expression in Raji cells.
- * The study provides insights into the molecular mechanisms of maslinic acid's anti-cancer potential.
- * Temporal gene expression analysis reveals dynamic pathway regulation by maslinic acid.
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