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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
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A high-throughput screening platform for pigment regulating agents using pluripotent stem cell-derived melanocytes.

Valentin Parat1, Brigitte Onteniente1, Julien Maruotti1

  • 1Phenocell SAS, Grasse, France.

Experimental Dermatology
|December 21, 2020
PubMed
Summary

This study introduces a new assay using stem cell-derived melanocytes and flow cytometry to measure melanin content. This method efficiently identifies agents that regulate skin pigmentation.

Keywords:
flow cytometryhigh-throughput screeninginduced pluripotent stem cellsmelanogenesispigment

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Dermatology

Background:

  • Melanin content in melanocytes is crucial for skin pigmentation and protection.
  • Identifying agents that modulate melanogenesis is important for cosmetic and therapeutic applications.
  • Existing methods for assessing melanin content can be laborious and time-consuming.

Purpose of the Study:

  • To develop a simple, high-throughput assay for quantifying melanin content in human melanocytes.
  • To identify novel pigment-regulating agents using this assay.
  • To establish a reliable method for screening modulators of melanogenesis.

Main Methods:

  • Utilized induced pluripotent stem cell-derived melanocytes.
  • Employed high-throughput flow cytometry based on forward light-scatter characteristics.
  • Correlated light scatter properties with cellular melanin content.
  • Performed orthogonal validation using standard melanin quantification techniques.

Main Results:

  • Developed a straightforward assay correlating forward light scatter with melanin levels.
  • Successfully validated the assay using a library of 80 small molecules.
  • Identified a confirmed hit compound that modulates melanin content.
  • Demonstrated the assay's utility in identifying modulators of melanogenesis.

Conclusions:

  • The described flow cytometry assay is an effective tool for assessing melanin content in human melanocytes.
  • This method offers a high-throughput and efficient approach for screening pigment-regulating agents.
  • The assay has the potential to accelerate the discovery of compounds influencing melanogenesis.