Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glucose Homeostasis: Regulation of Blood Glucose01:02

Glucose Homeostasis: Regulation of Blood Glucose

3.2K
Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
3.2K
Glucose Absorption Into the Small Intestine01:26

Glucose Absorption Into the Small Intestine

33.9K
Complex carbohydrates consumed cannot be absorbed into the small intestine in their original form. First, they must be hydrolyzed to a monosaccharide form such as glucose or galactose. These monosaccharides are then transported across the intestinal membrane and into the blood via transcellular transport. The intestinal epithelial cells allow the movement of these monosaccharides with a defined 'entry' through membrane transporter proteins present on their apical membrane and...
33.9K
Glucose Transporters01:27

Glucose Transporters

26.7K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
26.7K
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

1.8K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
1.8K
Membrane Proteins01:30

Membrane Proteins

28.1K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
28.1K
Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

873
The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
873

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longitudinal Trends of Salivary Oxidized Thymosin β4 and β10 in Preterm Infants with Bronchopulmonary Dysplasia.

Children (Basel, Switzerland)·2026
Same author

Postmortem Oxycodone Toxicology: A Systematic Review and Meta-Analysis of Concentrations and Interpretative Markers.

Molecules (Basel, Switzerland)·2026
Same author

The endosteal niche regulates breast cancer cell dormancy in bone: identification of new molecular determinants.

Bone research·2026
Same author

Effect of α-Synuclein Overexpression on NAPP-129 and TLQP-62 in Rat Brain and Plasma.

Medical sciences (Basel, Switzerland)·2026
Same author

CCND3 Suppression Ameliorates β-Thalassaemia in a Murine Disease Model: A Potential Therapeutic Strategy.

Cells·2026
Same author

Effects of the STriatal Enriched Tyrosine Phosphatase (STEP) Inhibitor TC-2153 on Hippocampal Long-Term Depression and Synaptic Transmission: Paradoxical Effect on Phosphatase Activity and Role of Adenosine.

CNS neuroscience & therapeutics·2026

Related Experiment Video

Updated: Nov 24, 2025

Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy
07:07

Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy

Published on: August 3, 2021

3.0K

TLQP-21 changes in response to a glucose load.

Giulia Corda1, Barbara Noli1, Barbara Manconi2

  • 1Department of Biomedical Sciences, University of Cagliari, Monserrato, CA, Italy.

Tissue & Cell
|December 21, 2020
PubMed
Summary

The peptide TLQP-21 is released from pancreatic islets during hyperglycemia to potentially enhance insulin secretion. Its levels in plasma increase after glucose administration, suggesting a role in glucose regulation.

Keywords:
EndocrineGlucoseInsulinMetabolismTLQP-21VGF

More Related Videos

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
08:03

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Published on: June 25, 2017

20.4K
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
10:35

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

Published on: April 6, 2022

3.0K

Related Experiment Videos

Last Updated: Nov 24, 2025

Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy
07:07

Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy

Published on: August 3, 2021

3.0K
Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
08:03

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Published on: June 25, 2017

20.4K
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
10:35

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

Published on: April 6, 2022

3.0K

Area of Science:

  • Endocrinology
  • Metabolism
  • Molecular Biology

Background:

  • The peptide TLQP-21 is known to enhance glucose-stimulated insulin secretion.
  • Investigating the endogenous response of TLQP-21 to glucose is crucial for understanding its physiological role.

Purpose of the Study:

  • To investigate the endogenous response of the TLQP-21 peptide to glucose administration in vivo.
  • To determine the localization of TLQP-21 and its receptors (gC1q-R and C3a-R1) within the pancreatic islets.
  • To analyze the changes in TLQP-21 levels in both pancreatic tissue and plasma following a glucose challenge.

Main Methods:

  • Mice were administered glucose or saline and sacrificed at 30 and 120 minutes.
  • TLQP-21 and its receptor expression were analyzed using immunohistochemistry, ELISA, and HPLC.
  • Pancreatic tissue and plasma samples were used for peptide and receptor analysis.

Main Results:

  • TLQP-21 immunoreactivity was observed in insulin-, glucagon-, and somatostatin-containing cells within the pancreas.
  • Glucose administration led to a decrease in pancreatic TLQP-21 immunoreactivity and an increase in plasma TLQP-21 levels.
  • Both gC1q-R and C3a-R1 receptors were localized in TLQP-21-expressing islet cells, with distinct distribution patterns.

Conclusions:

  • Hyperglycemia triggers the release of TLQP-21 from pancreatic islets, suggesting a role in enhancing insulin secretion.
  • TLQP-21 may also exert autocrine effects regulating insulin storage and secretion.
  • The findings highlight TLQP-21 as a potential regulator of glucose homeostasis.