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Related Concept Videos

Proofreading01:31

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Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
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Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
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Related Experiment Video

Updated: Nov 24, 2025

Transcorporal Artificial Urinary Sphincter Cuff Placement in a Case Requiring Revision for Urethral Atrophy
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Erratum.

Jing Zeng1, Tianping Du2, Yafeng Song3

  • 1Department of Infection Control, Taihe Hospital, Hubei University of MedicineShiyan, Hubei ProvinceP.R. China.

Oncology Research
|December 22, 2020
PubMed
Summary

Long noncoding RNA colon cancer-associated transcript 2 (CCAT2) is overexpressed in glioma and linked to poor survival. Reducing CCAT2 inhibits glioma growth, migration, and invasion, suggesting its potential as a therapeutic target.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in various cancers.
  • The role of lncRNAs in glioma tumorigenesis remains largely unexplored.
  • Colon cancer-associated transcript 2 (CCAT2) is a lncRNA with known roles in other cancers.

Purpose of the Study:

  • To investigate the biological function and clinical significance of lncRNA CCAT2 in glioma.
  • To determine the association of CCAT2 expression with glioma grade, size, and patient survival.
  • To explore the potential of CCAT2 as a therapeutic biomarker for glioma.

Main Methods:

  • Quantitative real-time PCR to assess CCAT2 expression in glioma tissues and cell lines.
  • Correlation analysis between CCAT2 levels and clinicopathological features (tumor grade, size).
  • In vitro experiments involving CCAT2 knockdown to evaluate effects on glioma cell proliferation, migration, invasion, apoptosis, and epithelial-mesenchymal transition (EMT)-associated gene expression.

Main Results:

  • lncRNA CCAT2 was significantly overexpressed in glioma tissues and cell lines compared to normal controls.
  • High CCAT2 expression correlated with advanced tumor grade and larger tumor size.
  • Patients with high CCAT2 levels exhibited significantly poorer overall survival.
  • Knockdown of CCAT2 suppressed glioma cell growth, migration, and invasion, and induced apoptosis.
  • CCAT2 was found to regulate the expression of EMT-associated genes.

Conclusions:

  • lncRNA CCAT2 is a crucial oncogenic factor in glioma progression.
  • CCAT2 overexpression is associated with aggressive tumor characteristics and poor prognosis in glioma patients.
  • CCAT2 represents a promising biomarker for glioma diagnosis, prognosis, and therapeutic strategies.