Related Experiment Video
Updated: Nov 24, 2025
08:48
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
3.0K
Quantitative evaluation of human sperm viability using MTT assay: A laboratory study.
Hamid Reza Momeni1, Mohammad Hussein Abnosi1, Najmeh Eskandari1
1Department of Biology, Faculty of Science, Arak University, Arak, Iran.
International Journal of Reproductive Biomedicine
|December 22, 2020
Summary
The MTT assay reliably assesses human sperm viability by measuring mitochondrial activity. This method shows a strong correlation with sperm motility and can predict fertilization potential.
Area of Science:
- Reproductive Biology
- Cellular Biology
- Biochemistry
Background:
- The MTT assay quantifies cellular mitochondrial activity, commonly used for assessing cell proliferation and viability.
- Evaluating human sperm viability is crucial for reproductive medicine and fertility assessments.
Purpose of the Study:
- To evaluate the efficacy of the MTT assay for determining human sperm viability.
- To analyze the correlation between sperm viability and motility.
Main Methods:
- Human ejaculated semen samples (n=56) were analyzed.
- Sperm viability was assessed using a quantitative MTT assay.
- Sperm motility was evaluated according to World Health Organization guidelines.
Main Results:
- A significant positive correlation was observed between the MTT reduction rate and the percentage of viable spermatozoa.
- Pearson's correlation analysis confirmed a significant association between sperm viability and motility.
Conclusions:
- The MTT assay, based on mitochondrial functionality, is a dependable method for assessing human sperm viability.
- This assay holds potential as a diagnostic tool for predicting sperm fertilizing capacity in clinical applications.
