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Imaging the future: the emerging era of single-cell spatial proteomics
Indranil Paul1, Carl White1, Isabella Turcinovic1
1Center for Network Systems Biology, Department of Biochemistry, Boston University, MA, USA.
The FEBS Journal
|December 22, 2020
Summary
Cellular compartmentalization is key for biological processes. This review explores technologies for single-cell spatial proteomics to understand cell function and disease.
Area of Science:
- Cell Biology
- Proteomics
- Systems Biology
Background:
- Cellular proteomes are compartmentalized within organelles and subcellular structures.
- Protein localization and trafficking are crucial for regulating cellular processes like signaling and cell death.
- Protein mis-localization can lead to various pathologies.
Purpose of the Study:
- To review platform technologies for subcellular proteomics and high-throughput systems biology.
- To provide mechanistic insights into fundamental cell biological processes.
- To highlight the importance of single-cell measurements for understanding biological heterogeneity.
Main Methods:
- Review of diverse platform technologies for subcellular proteomics.
- Discussion of high-throughput systems biology approaches.
- Analysis of methods enabling single-cell spatial proteomics.
Main Results:
- Compartmentalization enables efficient and isolated biochemical processes.
- Dedicated trafficking mechanisms ensure correct protein localization.
- Single-cell spatial proteomics is an emerging field with significant potential.
Conclusions:
- Understanding subcellular protein organization is vital for cell biology and disease research.
- Advancements in single-cell spatial proteomics will offer deeper mechanistic insights.
- Developing robust single-cell spatial proteomics tools is a critical future direction.
