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Agaricus bisporus Crude Extract: Characterization and Analytical Application
Maria A Morosanova1, Tatyana V Fedorova2, Alexandra S Polyakova1
1Analytical Chemistry Division, Chemistry Department, Lomonosov Moscow State University, 119234 Moscow, Russia.
Molecules (Basel, Switzerland)
|December 23, 2020
Summary
Crude Agaricus bisporus extract (ABE) effectively substitutes purified tyrosinase for analytical applications. This mushroom extract demonstrates similar substrate specificity and affinity, enabling accurate quantification in food supplements, medical diagnostics, and environmental monitoring.
Area of Science:
- Biochemistry
- Enzymology
- Analytical Chemistry
Background:
- Mushroom tyrosinase is crucial for various biochemical processes.
- Purified enzymes can be costly and unstable for routine analysis.
- Agaricus bisporus extract (ABE) offers a potential alternative source of tyrosinase activity.
Purpose of the Study:
- To characterize crude Agaricus bisporus extract (ABE) for tyrosinase activity.
- To evaluate ABE's substrate specificity and affinity compared to purified tyrosinase.
- To establish analytical procedures using ABE for various applications.
Main Methods:
- Preparation and characterization of crude Agaricus bisporus extract (ABE).
- Confirmation of mushroom tyrosinase (PPO3) using 2D electrophoresis and MALDI TOF/TOF MS.
- Substrate specificity analysis using 19 phenolic compounds.
- Development and application of spectrophotometric determination methods.
Main Results:
- ABE contains mushroom tyrosinase (PPO3) along with other enzymes like glucosidases and mannosidases.
- ABE exhibited substrate specificity and affinity comparable to purified Agaricus bisporus tyrosinase.
- Analytical procedures were developed for quantifying l-tyrosine, phenol, catechol, caffeic acid, chlorogenic acid, and l-DOPA.
Conclusions:
- Crude Agaricus bisporus extract (ABE) can serve as a viable and cost-effective substitute for purified tyrosinase.
- ABE-based analytical methods are applicable for quality control in food supplements, medical diagnostics, and environmental analysis.
- This study highlights the potential of using crude enzymatic extracts in routine analytical settings.
