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Optimization of Adsorbent Layer Type and Developing Solvent in Coccidiostats Sample Preparation with Procedure of
Maciej Jan Rybicki1, Anna Klimek-Turek1, Tadeusz Henryk Dzido1
1Department of Physical Chemistry, Medical University of Lublin, ul. Chodźki 4a, 20-093 Lublin, Poland.
Molecules (Basel, Switzerland)
|December 23, 2020
Summary
This study introduces Solvent Front Position Extraction, a new method for preparing samples to detect coccidiostat residues in food. This technique improves the analysis of these veterinary drugs, ensuring food safety.
Area of Science:
- Analytical Chemistry
- Food Safety
- Veterinary Pharmacology
Background:
- Coccidiostats are widely used in animal breeding to combat coccidiosis.
- Residues of these drugs in food pose potential risks to human health.
- Regulatory bodies like the EU have set concentration limits for coccidiostats in animal feed and food products.
Purpose of the Study:
- To develop and validate a robust sample preparation method for coccidiostats.
- To establish common conditions for isolating eight different coccidiostats from complex biological matrices.
- To enable accurate quantitative determination of specific coccidiostats in feed premixes.
Main Methods:
- Solvent Front Position Extraction (SFPE), a planar chromatography technique.
- Coupling SFPE with Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) for sensitive detection.
- Optimization of isolation conditions for coccidiostats with varying retention characteristics.
- Utilizing a semi-automatic device for chromatogram development.
Main Results:
- Effective isolation of eight coccidiostats from biological sample components was achieved.
- The method successfully isolated monensin and salinomycin from premix samples.
- Quantitative determination of monensin and salinomycin was performed with high accuracy.
- The use of a semi-automatic device enhanced the reliability and efficiency of the analysis.
Conclusions:
- Solvent Front Position Extraction is an effective sample preparation technique for coccidiostats.
- The developed method, coupled with LC-MS/MS, allows for reliable quantification of coccidiostats in biological samples.
- This approach contributes to improved monitoring of coccidiostat residues, enhancing food safety.
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