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Updated: Nov 24, 2025

Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status.

Céline Ducloux¹, Bruno You¹, Amandine Langelé^1,2

¹Laboratoire Français du Fractionnement et des Biotechnologies (LFB), 3 avenue des Tropiques, Les Ulis, BP 305, 91958 Courtabœuf Cedex, France.

|December 23, 2020

Summary

Human parvovirus B19 (B19V) causes diverse diseases. A new cell-based method using UT7/Epo-STI cells accurately quantifies infectious B19V units, improving detection and safety.

Keywords:

B19 parvovirus cell cycle detection erythroid cells permissivity

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Last Updated: Nov 24, 2025

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Area of Science:

Virology
Cell Biology
Infectious Diseases

Background:

Human parvovirus B19 (B19V) causes a spectrum of diseases, from mild childhood infections to severe anemia and fetal hydrops.
Current methods for detecting B19V contamination, like nucleic acid testing, do not accurately reflect viral infectivity.
A lack of appropriate cellular models hinders the detection of infectious B19V units.

Purpose of the Study:

To develop an improved cell-based method for detecting and quantifying infectious human parvovirus B19 units.
To identify sensitive cell lines that support B19V infection and replication.
To establish a reliable assay for assessing B19V infectivity.

Main Methods:

Screening of various cell lines for susceptibility to B19V infection.
Development of stable UT7/Epo-STI cell line clones with varying permissiveness to B19V.
Evaluation of the correlation between B19V infectivity and the cell cycle stage (S/G2/M).

Main Results:

The UT7/Epo-STI cell line demonstrated high sensitivity and ease of use for B19V infection.
Stable clones, particularly B12 and E2, exhibited enhanced sensitivity to B19V compared to existing models.
A direct correlation was observed between B19V infectivity and the S/G2/M cell cycle stage.

Conclusions:

Cell cycle status is crucial for B19V sensitivity.
The developed UT7/Epo-STI cell-based method offers a straightforward and sensitive approach for quantifying B19V infectious units.
This new method has significant implications for blood safety and understanding B19V pathogenesis.