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Related Concept Videos

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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Related Experiment Video

Updated: Nov 24, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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RNA-Centric Methods: Toward the Interactome of Specific RNA Transcripts.

Cathrin Gräwe1, Suzan Stelloo1, Femke A H van Hout1

  • 1Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, 6525 GA Nijmegen, The Netherlands.

Trends in Biotechnology
|December 23, 2020
PubMed
Summary

Identifying specific RNA-protein interactions is crucial for understanding cellular processes. This review covers methods to map these interactions, focusing on specific RNA transcripts and future directions like CRISPR-RNA systems.

Keywords:
RNA affinity purificationsRNA-protein interactionsmass spectrometryproximity-dependent biotinylation

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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • RNA-protein interactions are vital for cellular functions and implicated in various diseases.
  • Global studies have identified numerous RNA-binding proteins, but specific RNA-protein binding remains largely uncharacterized.

Purpose of the Study:

  • To review methods for identifying RNA-protein interactions.
  • To highlight strategies for mapping the interactome of specific RNA transcripts.
  • To discuss future directions, including the use of CRISPR-RNA systems.

Main Methods:

  • Overview of techniques for RNA-protein interaction identification.
  • Focus on methods providing transcript-specific interaction data.
  • Discussion of emerging technologies like CRISPR-based approaches.

Main Results:

  • Current methods offer varying degrees of specificity in identifying RNA-protein binding partners.
  • Transcript-specific approaches are essential for detailed functional studies.
  • CRISPR-RNA targeting presents a promising avenue for investigating endogenous interactions.

Conclusions:

  • Advancements in RNA-protein interaction mapping are crucial for understanding gene regulation and disease mechanisms.
  • Future research should focus on high-resolution, transcript-specific interaction studies.
  • CRISPR-RNA technology holds significant potential for dissecting endogenous RNA-protein complexes.